Use a new glucose strip and record Data: Conclusion: According to my data, pH levels do, in fact, affect the results of Lactex. I say this because in my experiment, pre-enzyme, my solutions were all negative except for the soda. I used the test strips to determine if they were negative or positive solutions. After I put in the enzyme, Lactex, my results changed. Vinegar, Antacid, and Soap all became positive solutions after I put in the enzyme.
BACKGROUND: For these experiments a 96-well plate was used. Each chemical listed would be put into their own well of 2 drops of each chemical unless instructed otherwise. After the chemicals were each dropped into the wells they were stirred with a different toothpick each time (to not cause cross contamination) and then was observed over a white piece of paper and then a black piece of paper. For each reaction I recorded the well number and took detailed notes on the reactions observed. After the first two tests done with BTB I hypothesized that acids would turn BTB yellow and that bases would remain blue.
At the high pHs, the enzyme lactase is denatured and is no longer able to break down the sugar lactose in milk. Therefore, no glucose is present and the test strips remain blue. . The optimum range for the activity of lactase would be considered pH 2-7. The water control tube should match the tube with a
For the food test that use to determine the of presence protein we use Biuret test instead of Million’s Test. The reason why we are choosing the Biuret test is because the Millon’s Test cannot have positive result on specific protein such as gelatine due to the gelatin had no tyrosine beside the Million’s Test will show positive result on every substance which had the tyrosine including non protein substance. So we used albumin solution and it will only show positive result on protein only. Objective -To determine the presence of reducing sugar by carrying of Benedict’s test -To determine the presence of non reducing sugar by carrying out Hydrolysis process by using dilute hydrochloric acid. -To show the presence of starch by carrying out iodine test.
Chapter 10 Lipids Multiple Choice Questions 1. Structural lipids in membranes Pages: 343-345 Difficulty: 2 Ans: A Which of the following statements concerning fatty acids is correct? A) One is the precursor of prostaglandins. B) Phosphatidic acid is a common one. C) They all contain one or more double bonds.
BIOL-110 Elements of Biology Assignment 1 Student Name: Part A: Multiple Choice Questions and Fill in the Blanks 1) A covalent chemical bond is one in which * c) Outer-shell electrons of two atoms are shared 2) Which of the following is not a weak molecular interaction? * a) A covalent bond 3) Polysaccharides, lipids, and proteins are similar in that they * b) Are synthesized from monomers by dehydration reactions. 4) Which of the following is a compartment that often takes up much of the volume of a plant cell? * b) Vacuole 5) The presence of cholesterol in the plasma membranes of some animals * a) Enables the membrane to stay fluid more easily when cell temperature drops 6) Which of the following types of molecules are the major structural
In the toxic mussels, the visible light absorption spectrum revealed a pattern that was characteristic of phytoplankton pigments. With further investigation, the pigments were found not to be poisonous though, and the aqueous layer consisted of the toxin. Column chromatography was used to separate the layer into organic acids and bases. Acids that were ionized quickly passed through because the resin called XAD-2 would not hold them in their polar ionized form. Out of all the acids that passed through, only one was found to be toxic.
By looking at the spread sheet it conveyed that all 3 bacterials were negative for the oxidase test. So, I assumed that if the oxidase test performed came back negative as well, this would verify that I chose wisely. The oxidase test would also detect if the bacteria produced cytochrome oxidase, it is also used to segregate between aerobic gram-negative organisms. The last experiment set up on this day was the fluid thioglycollate medium (FTM) so that I could verify if my unknown bacteria was a aerobic or anaerobic
Most differential stains have a challenge step that follows staining with a primary dye. In the Gram stain the challenge step is a rinse with either ethanol or acetone (either may be used). This step dehydrates and tightens the cell wall of Gram positives (mainly peptidoglycan) such that the rinse does not enter the cell. Gram negatives have mainly a lipid cell wall (even though they do contain peptidoglycan) that allows the challenge rinse to penetrate the cell and rinse out the crystal violet-iodine complex rendering the Gram negative cell colourless. Thus, the Gram negative cells must be stained to be seen, and this is done with the counter stain.
Proteins are extracted from animals and humans as a mixture in a serum of body fluids. When immunologists want to study a specific protein, like an antibody, hormone, or enzyme, they need to separate it from the mixture. The technique is used for the separation of sugar, Polypeptides, Proteins, Polystyrenes, Silicones, Liquids, Butyl rubbers and polyethylenes. Types: MEC is a one of several types of HPLC. There are basically two types of MEC.