Title and Identifiers: Observations of Chemical Changes by the use of acids and bases. Abstract: To observe the small changes that occur in chemical reactions and attempt to interpret the changes of the atoms and molecules that allow for these small changes to happen; and to associate these chemical properties with household products. We will also learn how to separate mixtures. Experiment Procedures: 24-well plate is used as a convenient holder for the pipets of chemicals. 96-well plate is used for mixing the chemicals.
Ocean County College Department of Chemistry [Observations of Chemical Changes] Submitted by Andrew Grimm Date Submitted: 5/31/14 Date Performed: 5/31/14 Lab Section: Chem-181DL2 Course Instructor: Amal Bassa Purpose The main goal in this experiment is to observe the properties of simple chemical reactions, and relating their chemical properties to household products. Procedure To begin this experiment I placed two drops of NaHCO3 into well A1 of the 96-well plate, and then added two drops of HCl into well A1. Next I placed two drops of HCl into well B2, and added two drops of BTB, and observed the color. Then I placed two drops of NH3 into well C1 and added one drop of BTB, and observed the color. Next I added two drops of HCl into well D1, and then added two drops of blue dye, and recorded what I saw.
Objective: To analyze each solution in order to determine if organic compounds; carbohydrates, proteins, and lipids are present. Hypothesis: If reactions have occurred once each agent has been applied then, this will show weather carbohydrates, proteins or lipids are present. Abstract: My experiment was based on analyzing the following organic compounds: Carbohydrates, proteins and lipids. During my analysis I ran test to gather information containing different color reactions using Benedicts test for reducing sugars, Iodine test for starch, the Biuret test for protein, and the Sudan IV test/Grease Spot test for lipids. After testing the following solutions: onion juice, potato juice, sucrose solution, egg albumen, honey, amino acid solution, distilled water, protein solution, salad oil, known lipid solution, and the unknown solution (3), here’s what I concluded: While some solutions have no reaction to the agent being applied, some color reactions have occurred, hereby leaving me to assume that that carbohydrates, proteins and lipids are present.
Methods My group used the method of titration to test whether the Vitamin C and lime juice nutritional facts where correct from their manufacture. First, we started with the standardized solutions, NaOh and HCl, to get familiar with how titrations work and start the titration of potassium hydrogen phthalate (KHP). We begun by gathering up all the necessary materials needed for the titration including: the general laboratory glassware from the bins, standard safety equipment, 50mL burette, phenolphthalein, NaOh (3 mol/L), HCl (3mol/L), KHP, ascorbic acid, and lime juice. The standardization of NaOh came first, which started by gathering the KHP and putting 1.5 g into 3 flasks with 50 mL of water in each. After we added the KHP to the 50 mL of water, we placed each one of the flasks onto a stir plate with a magnet in each flask so we achieved maximum stirring.
As a more polar solvent is use to push the different rings of pigment, these are collected in their own test tubes to then be run in a TLC which will determine the polarity using the Rf values and then comparing them to the table in the organic lab manual ones. This is important because we want to know what some things are composed of and by using the polarity in different components makes it easier to determine them. The gain from this experiment is determining the different pigment of spinach and by doing so comparing the polarity of each of them. Then determine why that could be by looking at the structures of the pigments separated. Discussion: The first thing done in
We also concluded that the McMush did have starch because when it was mixed with the solutions, it turned completely black which matched the data from our previously data table. In addition for testing the McMush for starch, chloride, protein, or glucose; we checked the lipid content by putting a little amount of it on a paper towel and letting it dry. Once dry, we help it up to a light and saw little chunks in the paper towel and areas were the paper towel was translucent. This proved there was fat within the McMush. From this we gathered that there was a lot of fat within the McMush.
In this lab we will investigate how amylase acts on starch, lipase on lipids, and trypsin on protein. Amylase is found in our saliva and breaks down starch for digestion. We will use iodine in the experiment to detect the presence of starch. When iodine makes contact with starch, its natural reddish-brown color turns dark purple. We will detect how long it takes for amylase to react and break down the starch.
Sodium Hydroxide + Hydrochloric Acid Sodium Chloride + Water NaOH(aq) + HClaq → NaCl(aq) + H2O(l) Variables Independent The independent variable of the experiment was the amount of sodium hydroxide that we added to the acid. To keep the variable controlled we would measure 1 ml of the sodium hydroxide and pouring that to the hydrochloric acid. Dependent The dependent variables of the experiment were the temperature and the pH number of the mixture. To control the pH and temperature use the electronic probe and data logger. Controlled The controlled variables of the experiment were: A.
Observation 7 Hydrogen Gas was being performed. Observation 8 Zinc Chloride White bubbles started, and still has solid. Discussion: During the experiment we had to pour into the test tube 5 ML of Copper Sulfate Solution. Once this was done it started turning into a blue green color. Next, Zinc and Copper Sulfate combined which there was gas bubbles and the color changed into blue green color.
You know what color phenolphthalein and bromothymol blue turn when testing an acid or a base. Use the empty pipet in the Auxiliary Supplies Bag to test several (at least 3) household items including household cleaning products with bromothymol blue. Rinse the pipet well before using it on the next household chemical. When finished with this experiment rinse the pipet well and return it to the Auxiliary Bag for use in future experiments. Name the items tested and record their results.