It is for this reason that Identification of bacteria is very important in the many areas of microbiology (Singh, 2009). There are many reasons for identifying an unknown bacterium. The reasons range from medical purposes, such as determining if the unknown could cause ailments in living things or knowing what microorganisms are needed to make antibiotics to other purposes such as knowing the exact microorganism has to be used to make certain foods (Burton, 2003). Throughout this report we will be addressing the differences in methods of identifying unknown microorganisms and differentiate between the results of these techniques by explaining how each outcome was achieved. The main aim of these experiments is to determine whether the unknown microorganisms are of Gram-Positive or Gram Negative nature and ultimately to determine exactly what kind on microorganism the unknowns are.
MacConkey agar was used to help determine this. The last test that helped determine this bacteria was the Methyl Red Voges-Proskauer broth. This test came back negative. In conclusion, I believe that the unknown bacteria number two to be Enterobacter aerogenes. In comparison of the descriptive chart and the comparative analysis sheet, 18 out 18 of the tests performed were alike.
I added a few drops of 3% Hydrogen Peroxide and it resulted in bubble formation. The positive gram stain and positive catalase test indicated that the organism belonged to the Staphylococcus Genus, which meant it was now time to pinpoint the species based on differential and selective biochemical tests. To ensure that my genus identification was correct I performed a Bile Esculin Slant with a fish tail streak on my unknown bacteria. This test resulted in neither growth nor a black precipitate; therefore I could conclude that my unknown was not Streptococci or Enterococci but indeed Staphylococcus. Next I observed the isolation streak on my Blood Agar Plate and found pinpoint, round, entire and flat colony morphology as well as an alpha hemolytic reaction pattern, indicating red blood cell ion leakage which is characteristic of S. epidermidis.
The purpose of these tests were to determine how the bacterium reacted to glucose with and without oxygen. My findings reported a gram stain negative along with positive tests for glucose oxidation and fermentation. Next I performed an Indole Production Test. This test determines whether the microbe produces indole from the amino acid tryptophan. The results from this test were negative.
pGLO Transformation Scientific Report BY : Tom riddle pGLO Transformation Scientific Report BY : Tom riddle Introduction The aim was to conduct an experiment that genetically transformed bacteria with Biorard pGLO plasmid. The pGLO plasmid has ability for the production of the green Fluorcent protein (GFP) (Biorard2000) which is also found in jellyfishes. The experiment conducted was to transform the e-coli bacteria with jellyfish gene that code for green fluorescent protein (GFP). During the experiment procedures were undertaking for the genetic transformation, at the end if done correctly the new developed jellyfish gene in the bacteria will glow under UV light. In this experiment there were main 4 hypothesis made according to each agar plate each with a different types of genes on them.
Calonna Taylor Professor Osborne BSC2010C 9 February 2014 Beyond The Cells Purpose: The purpose of this lab report is to become familiar with the use of a compound microscope and the Gram Stain. Hypothesis: If you do not know how to use a Microscope or how to Gram Stain, then your results of your lab will come out inconclusive or just wrong. Part A: Cell Structures Materials & Methods: Materials: * Colored Pencils * Paper * Access to a scanner Methods: 1. View video “Using a Microscope.” 2. Look at the slides and identify the cell structure within.
As a more polar solvent is use to push the different rings of pigment, these are collected in their own test tubes to then be run in a TLC which will determine the polarity using the Rf values and then comparing them to the table in the organic lab manual ones. This is important because we want to know what some things are composed of and by using the polarity in different components makes it easier to determine them. The gain from this experiment is determining the different pigment of spinach and by doing so comparing the polarity of each of them. Then determine why that could be by looking at the structures of the pigments separated. Discussion: The first thing done in
Subject: Separation of unknown organics by flash column chromatography Introduction Column chromatography is a process that is used as a purification technique. The purpose of this lab is to isolate desired compounds from a mixture. The goal of this experiment is to apply column chromatography to separate two compounds and analyze their purity. It will be accomplished by running the mixture to be analyzed through the top of the column tube and then find purities by a TLC plate after the mixture has been separated. Reactions: Results: Calculations: Discussion: The TLC plate had 5 circles, indicating the 5 compounds, when it was under the UV lamp.
Purpose (5 points): The purpose of this lab is to learn how to extract DNA and to analyze extracted DNA. This lab allows the conductor of the lab to analyze the steps taken to extract the DNA and realize the purpose of each step. This lab activity teaches one how cell barriers can be broken. Hypothesis: If the enzyme, alcohol, detergent, alcohol, and salt are all used accordingly to extract the DNA from the split peas, then a small amount of the DNA will separate from the solution, looking like long thin strands. DNA is insoluble in alcohol, but soluble in water, so this experiment will test this scientific principle of alcohol.
Unknown Laboratory Report Identification of Unknown # 15 Ashley Grace C. May 4th 2011 Elizabeth Russo Microbiology 274 Spring 2011 INTRODUCTION There are many reasons behind the importance of identifying unknown bacteria. Reasons range from finding out how it can impact humans and the cause of a specific infection and diseases, to research for potential new pharmaceuticals to cure diseases. It is also important as bacteria are always mutating and may identify new strains that cause diseases or identify microbes that have become resistant to antibiotics. We can determine an unknown microbe by performing a variety of physiological and biochemical tests. MATERIALS AND METHODS Materials and methods are explained in chronological order.