. Experiment #2: Determining Effect of pH on Lactase Activity will involve students testing a range of different pH solutions mixed with cow milk to see if there is a change in the lactase activity. Access student prior knowledge on what conditions most affects enzyme activity (pH and temperature). Discuss what neutral, acidic, and basic (or alkaline) pH values are and where they are found in the pH range (0-14). Review the effect of pH on enzyme function.
Another source of rennin is junket tablets, which are used in setting milk into a solid junket or dessert. Junket tablets are used as a source of rennin in this experiment. Materials (per group) (This experiment may be done in groups of four. Each student can look after two temperature set-ups.) » 2 Bunsen burners »2 tripods » 2 wire gauzes » 2 × 150 mL beakers » 2 × 250 mL beakers for 70°C and 80°C water baths » 5 Styrofoam cups for unheated water baths » 8 thermometers 0–100°C » 16 test tubes in a rack » 10 mL measuring cylinder » junket tablet » 50 mL milk » Crushed ice » dropper » plastic spoon » marking pen » Distilled water Note: Thermostatically controlled water baths may be used if available.
Materials & Methods Materials: · Scale · 4 6” Dialysis Tubing · 4 Transfer Pipets · Sugar · Scissors · Rubber Bands · 4 Same-Sized Coffee Cups · 250ml Graduated Cylinder · Tape Measure · Sauce Pan · 3 600ml Containers · Plastic Covering · Spoon Methods: 1.) Place 4 6” pieces of dialysis tubing into coffee cups full of tap with and leave them for two hours prior to beginning the experiment. 2.) As you wait, prepare your three sugar solutions. For the first solution, pour 5 grams of sugar into 250ml graduated cylinder and add water up to the 250ml mark.
When an indicators color changes it shows the presence of an organic compound. The purpose of using distilled water as one of the substances is that it is the control so you can see the differences. Also, in the substances distilled water was mixed in with them. The control group in part C is distilled water. The purpose of washing the test tubes thoroughly is so the chemicals don’t cross contaminate and affect the outcome of another section of the lab.
Add 5 drops of MgCl2 to wells C1 and C2, 10 drops to C3 and C4 5. Add 1 drop of phenolphthalein to wells C1-C4, stir with toothpick 6. Add 5 drops of NaOH to C1-C4, stir and record observations 7. Add 1 drop of HCl to C1 and stir, add more drops until color changes, record observations 8. Add 1 drop of Na2 EDTA to C2 and stir, add more drops until color changes, record observations 9.
Tablet | A report based on a scientific experiment designed to investigate and compare the rates at which different forms of aspirin dissolve. | 11-11-2013 | Introduction This experiment was designed to investigate and compare the rates at which different aspirin tablets dissolve in both Water (H2O [representing saliva]) and Hydrochloric Acid (HCl [representing the stomach’s acid]). The amount of Water and Hydrochloric Acid will be kept constant between tablets, and tests. Aim To investigate and compare the rates at which different Aspirin Tablets dissolve. The different kinds of Aspirin tablets are: * Enteric Coated Tablets * Capsules * Regular Tablets * Dissolving Tablets.
Next, we put starch solution and a couple drops of iodine into a reaction tube, and recorded the color. We then got another reaction tube, put starch solution and saliva in it, and then put it underwater in a machine for 30 minutes. After the 30 minutes, we removed the tubes from the machine and added a couple drops of iodine to the solution. Lastly, we compared the colors of the two solutions to each other. Sweetness perception is the ability to taste sweetness.
We pour a drop or two of water onto one piece of paper bag and another drop of oil onto a different piece of paper bag. After a couple of minutes we identify for lipids. If the paper bag leaves an oily spot then lipids is active. Expected Results- The hypothesis of all experiments were true. Protein is in fact active when the liquid turns purple due to biuret reagent.
affect the enzyme activity, to get enzymes working there should be proper ph. to avoid denaturing of enzymes, changes in pH alters ionization of charged amino acids. In an experiment to determine the pH range over which the enzyme catechol oxidase is able to catalyze its substrate, this will also help determine the optimum pH in which enzymes work best. Materials • 7 test tubes • Test tube rack • Metric ruler • China marker • Wash bottle containing 1%
This happens because there is no insulin to move glucose out of your bloodstream and into your cells to produce energy. If your blood glucose levels become too high, you may experience hyperglycaemia. The symptoms are similar to the main symptoms of diabetes, but they may come on suddenly and severely. They include: * extreme thirst * a dry mouth * blurred vision * drowsiness * a frequent need to pass urine If left untreated, hyperglycaemia can lead to diabetic ketoacidosis, which can eventually cause unconsciousness and even death. Diabetic ketoacidosis occurs when your body begins to break down fats for energy instead of glucose, leading to a build-up of acids in your blood.