Certain enzymes are specific to pH levels at which they work best, and removing them from this environment may denature the enzyme. Much like pH levels, enzymes have an optimal temperature level, and again in a different temperature extreme, enzymes will denature. However, other than only environmental factors, enzymes can also have their reactions affected by both activators, which increase reaction rates, and inhibitors, which slow or stop reactions. In an experiment involving the unexpected lowering of pH in an enzyme's environment, we can see how the enzyme will become denatured because of this. Materials: 2A) Hydrogen peroxide, catalase solution, beakers/test tubes, pipette, ice for catalase, water for boiling, liver/potato tissue 2B) Hydrogen peroxide, water, sulfuric acid, potassium permaganate, beakers/test tubes, pipette 2C) Hydrogen peroxide, beakers/test tubes, sulfuric acid, potassium permaganate, pipette 2D)
FACTORS AFFECTING ENZYME FUNCTION LAB#1 Background information Enzymes are specialized class of protein that acts as catalysts for the chemical processes within our cell which may occur slowly. To speed up these chemical reactions and for proper function, each enzyme binds with a substrate. The substrate binds to the active site of the enzyme. This union of substrate to the active site lowers the activation energy. This energy reduction allows reactions to occur at faster rate.
Whereas the pH moves from a more acidic concentration to a more basic, with a constant concentration of enzymes, the absorbance increases, to acidic or basic the enzyme denatures. Introduction Enzymes are proteins that catalyze a chemical reactions. The ability for proteins to accomplish that depends on the three-dimensional shape, different enzymes have different shapes. Without enzymes catalyzing biochemical reactions, the reactions would happen to slowly, leaving the world a very different place. Enzymes work by lowering the activation energy of a reaction, by binding to a substrate and forming an enzyme-substrate complex.
This experiment is important to do because we must understand the way enzymes work in our own body, and so would be important to medical researchers trying to see how different things affects the body. Research Question: What is the effect of H2O2 concentration on float speed of Catalase soaked coffee filter? Hypothesis. Catalase is an enzyme that digests Hydrogen Peroxides into water and oxygen. Since the speed of an enzyme reaction relies on whether there is any substrate to react with, float speed should increase as substrate concentration increases.
We predicted a bell curve shape of a graph representing the change in temperature and the absorbance where the extreme temperatures will show that enzymes are unable to function properly and at high temperatures but they will operate best at their own optimal temperatures in between the extremes. Materials and Methods: The materials used in this experiment were: six glass cuvettes (one blank sample and five temperature labeled samples), micropipettes from Gilson (200 and 1000ul), a test tube holder, a flat surface, three water baths, a vortex mixer, reaction buffer, L-DOPA Sigma (D9628), Tyrosinase Sigma (D3824), and a spectrophotometer from Thermo Scientific. A control cuvette was created to set the spectrometer containing water, reaction buffer, L-DOPA, and Tyrosinase. Five cuvettes were incubated at 0, 25, 32, 42, and 60 degrees.The
Linea Eby January 31, 2011 Hao Xu Discovering the effects of Temperature Changes on the Tonoplasts and Plasma Membranes of Red Beet Root by Examining the Amount of Betacyanin Released from the Cell’s Vacuole Introduction: The semi-permeable plasma membrane of the cell serves many functions. It regulates the transportation of macromolecules in and out of the cell, holds the cell together, and protects the cell from its external environment (Biology 107 Lab Manual, 2011). When environmental conditions are altered outside the cell, the membrane shields the cells organelles from some of the damage. If environmental conditions are so great that they begin to damage the shielding properties of the membrane, the organelles will be subjected to the full extent of the detrimental conditions. Organelle damage can have a variety of consequences.
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We will be using the enzyme from the liver and placing it in hydrogen peroxide, this will create oxygen and water, we will measure the amount of oxygen produced we will catch and measure it in a measuring cylinder. The equation for the breakdown of hydrogen peroxide with the enzyme Catalase is: In our preliminary results we are trying to find out if there are any flaws in the method that we need to assess and possibly change. Also I need to try and keep all the relevant factors the same so that there is no variation in the results from outside elements such as a difference in heat on different days, we will try and control this as much as