Then proceed to decolorize with 95% ethanol until the ethanol running off the slide is clear. Briefly rinse with distilled water. Counter-stain with safranin (secondary stain) for 1 minute then rinse with distilled water and gently blot dry with a paper towel. Lastly observe slide using oil immersion with the 100x
Add 1 mL of deionized water to the small test tube containing the precipitate and mix it and centrifuge it for 60 seconds. Then, add the supernatant into the boiling test tube and repeat this step one more time with another 1 mL of deionized water. Acquire a pair of metal test tube holders and heat the boiling test tube to evaporate the water for 15 minutes. Let is cool after and weigh it. Then, calculate a percent yield of zinc iodide and write a balanced chemical equation and determine the limiting
Protects the bacteria from phagocytosis allowing the bacteria to stay in the body 6. pure culture 7. It is differential based on hemolysis of the agar. Hemolysis can be wide-narrow band beta, alpha, gamma, or none. 8. candle jar in microbiology is used for anaerobiosis in which a lit candle is placed in an air tight jar and if it went out, it would be because it used up all the available oxygen. 9. any streptococcus capable of hemolyzing erythrocytes, classified as α-hemolytic type, producing a zone of greenish discoloration much smaller than the clear zone produced by
What is the function of the iodine solution in the Gram stain? If it were omitted, how would staining results be affected? Iodine is used as a mordant, it combines with the dye in the Gram Stain. 2. What is the purpose of the alcohol solution in the Gram stain?
By looking at the spread sheet it conveyed that all 3 bacterials were negative for the oxidase test. So, I assumed that if the oxidase test performed came back negative as well, this would verify that I chose wisely. The oxidase test would also detect if the bacteria produced cytochrome oxidase, it is also used to segregate between aerobic gram-negative organisms. The last experiment set up on this day was the fluid thioglycollate medium (FTM) so that I could verify if my unknown bacteria was a aerobic or anaerobic
The reaction was monitored and removed from the heat once the effervescence ceased, and then was allowed to cool. Concentrated hydrochloric acid was added while heating and stirring the reaction for 15 minutes. The product was then allowed to cool and was vacuum filtered to a maroon color, while being washed with water, 6M hydrochloric acid, ethanol, and acetone. Then allow the product to dry for five minutes on the funnel. The resulting product was [Co(NH3)5Cl]Cl2 and yield was 4.453g (.017 mol, 84.8%) Distilled water (25mL) was added to concentrated ammonia (5mL) in a 125mL Erlenmeyer flask.
Obtain another 50-mL beaker and add 0.05g of Chromium(III) Nitrate to the beaker. Then, add 17 mL of distilled water and mix until the solid is dissolved. 3. Obtain a final 50-mL beaker and add 0.05 grams of the unknown substance to the beaker. Add 17 mL of distilled water and mix with a stir bar until the solid is completely dissolved.
Problem definition Design a process for production of dry penicillin crystals including block diagram with steps and required calculations Conditions 1) Penicillin production is inhibited by glucose 2) Dissociated penicillin dissolves well in water 3) Undissociated penicillin dissolves well in solvents 4) Penicillin can be hydrolyzed to unusable compounds in aqueous solutions with the rate proportional to concentration of H+ ions (acidity) 5) Mycelium cake is required to contain low concentration penicillin 6) Penicillin is a secondary metabolite and is only produced in stationary phase Penicillin production consists of a number of steps the first of which is fermentation. The purpose of the fermentation is to convert substrate to penicillin.