Osmosis Experiment Not Done

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Jamie Smith Lab 5:Osmosis and Diffusion Through a Semipermeable membrane The purpose of this lab is to simulate and observe the process of osmosis. Osmosis occurs when a membrane that has a level of permeability separates different concentrations of water. This experiment uses dialysis tubing to represent the differential permeability that a cell membrane exhibits when exposed to solutions with various tonicities. My hypothesis is if the 1% sugar solution inside simulated cell is placed in a coffee cup containing 1% sugar solution amount, there will be little osmosis because the outside of the simulated cell is of the same solution amount and isn’t higher in solute amount. If the1% sugar solution inside simulated cell is placed in a 50% sugar solution, water will move from the cell to the outside solution because the cell is higher in solute concentration. If the 50% sugar solution inside simulated cell is placed in a 1% sugar solution, then the water from the 1% sugar solution will move inside the simulated cell because the cell membrane is higher in solute concentration. If the 20% sugar solution inside simulated cell is placed in a 1% sugar solution, then there will be a slight osmosis effect of water moving from lower solute concentration of the cell to the higher solute concentration of the1% sugar solution. Materials and Methods: 1. I Cut four 6-inch pieces of dialysis tubing and soaked them in a coffee cup filled with tap water for 2 hours prior to my experiment. While I waited, I prepared the three sugar solutions using the following protocol: A.) I Added 5 grams of sugar to my 250ml graduated cylinder and then added tap water up to the 250ml mark. I placed a small piece of plastic wrap over the top of the graduated cylinder and mixed the sugar with the water. Then, I poured the contents into a small saucepan over the stove. I filled the graduated

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