Microbiology Unknown Report

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02 April 15, 2014 #7 Enterobacteriaceae, Escherichia coli Staphylococcaceae, Staphylococcus epidermidis ABSTRACT The objective of the Unknown Report is to identify two unknown bacterium based on a series of tests learned in the microbiology laboratory. The tests performed on organism A were Fluid Thioglycollate, Gram Stain, MR/VP, SIM, Simon Citrate, and LB plate. The results for organism A were Facultative Anaerobe for aerotolerance, Gram negative, MR positive and VP negative, the SIM test results were positive for Indole productivity and growth radiating but negative for sulfur production, Simon Citrate negative, and there was no red growth on the LB plate. These tests lead to the discovery of the bacterium Escherichia coli which…show more content…
He is the first scientist to record precise descriptions of fungi, protozoa, and bacteria (“Microbiology’s”). Louis Pasteur postulated the germ theory of disease which states “microorganisms are the causes of infectious disease. While Pasteur could not prove his theory, another scientist by the name of Robert Koch was able to. Procedures known as “Koch’s postulates” are principles that link microorganisms to specific diseases. The “Golden age” of microbiology occurred in the late 1800’s, this is when many infectious diseases were identified (cliffsnotes.com). After World War п, antibiotics were discovered and many diseases such as pneumonia, tuberculosis, meningitis, and syphilis declined (cliffsnotes.com). Studying bacteria is still very important in today’s society in order to prevent any epidemics from…show more content…
Add a drop of water using and inoculating loop to a clean glass slide. Then using a sterilized loop, smear the slide with the organism, let the slide dry completely and heat fix. Apply crystal violet stain (primary stain) for one minute, briefly rinse with distilled water. Apply iodine (mordant) for one 1 minute, briefly rinse with distilled water. Then proceed to decolorize with 95% ethanol until the ethanol running off the slide is clear. Briefly rinse with distilled water. Counter-stain with safranin (secondary stain) for 1 minute then rinse with distilled water and gently blot dry with a paper towel. Lastly observe slide using oil immersion with the 100x

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