In comparison of the descriptive chart and the comparative analysis sheet, 18 out 18 of the tests performed were alike. This data helped me identify this unknown bacteria and understand why it was that bacteria. Enterobacter aerogenes is a facultative anaerobic bacteria that is present in the intestinal tract of humans. This bacteria naturally occurs in soil and freshwater and is present on plants, sewage, and is an opportunistic pathogen present on human skin. Antibiotics can be used to treat the
A gel mould was prepared by sealing the open ends of Perspex through with autoclave tape and a sample comb positioned 0.5-1.0 mm above the surface of the trough. 2. A 1% agrarose gel was prepared by dissolving 0.5 g agrarose in 50 ml of 1X TAE in an Erlenmeyer flask or glass bottle with a loose fitting. 3. This was allowed to cool to approximately 55ºC and ethidium bromide (10mg ml⁻¹) was added to a final concentration of 0.5 μg ml⁻¹.
Abstract Isolation plays a key part in the identification of unknown microorganisms. To be able to successfully identify an unknown microorganism, it is necessary to have single, isolated colonies as references for the tests. Throughout this report, we will be examining the differences in unknown bacteria types, whether it is Gram Positive or Gram negative and will be looking at ways to distinguish the difference between the various unknown bacteria samples that are going to be used. The ultimate aim of this experiment is to correctly identify the different types of unknown microorganisms provided to us and differentiate their types. To do this, we will be using 8 different widely know techniques, including the use of streak plate, gram
Antibiotic Sensitivity Lab Report: Microbes with an Emphasis on E. col Microbiology 225 March 25, 2011 Abstract Antibiotics are a major contribution to our world, but with the misuse and overuse of them, many bacteria are starting to become more resistant. One of the studies that researchers use to measure resistance of microorganisms to antibiotics is the Kirby Bauer test. This test has enabled doctors to evaluate which antibiotic is best to treat different microbacteria. Within our microbiology class we were given four different bacteria to study resistance using the Kirby Bauer method. These organisms which are Staphylococcus epidermidis, Staphylococcus aureus, Pseudomonas aeriginosa, and Escherichia coli (E. coli) were tested against Penicillin, Tetracycline, Bacitracin, and Ciprofloxacin.
After improved understanding of the causes of disease there was understanding that you could cure a disease. Behring used this and Koch’s work to isolate anti toxins that would otherwise ,harm the body, to fight Diphtheria, Behring then found a way to inject it. Paul Ehrlich ( a member of Koch’s team) used his team to build on this work , he knew that certain dyes stained specific microbes (Koch’s work) furthermore with Behring’s work Paul tres to find a cure for syphillis a “magic bullet” that would only target the microbes and not the body. He managed to research seven years which was only made possible because of government funds. In 1909 Dr Hata had joined the research team and he reviewed the previous experiments.
In Microbiology Lab 3 I chose the unknown culture #14 and ultimately identified it as Staphylococcus epidermidis bacteria. On a microscopic level I found the organism to be gram positive with a coccobacillus shape and both tetrad and cluster cell arrangement. I performed an isolation streak with S. epidermidis on Nutrient Agar which resulted in pinpoint, round, entire, and flat macroscopic morphology. I took a loopful of the organism from the Nutrient Agar and placed it on a slide to perform the catalase test. I added a few drops of 3% Hydrogen Peroxide and it resulted in bubble formation.
Gently stir the pellets until the acid is dissolved by shaking the the apparatus. Lift the calorimeter lid and wash out its contents and the thermometer. Repeat this experiment using 50.0 mL of 1.0M acetic acid. Repeat experiment using 25.0 mL of each 2.0M sodium hydroxide and 2.0M acetic acid. Data Table(s): Reaction equation Mass of solid NaOH Initial Temp.
Once all the water from the beaker was gone the clamp was carefully replaced so there were no air bubbles in the tube. The tube connected to the aspirator was then removed and connected to the test tube. The gelatin capsule was placed in the tube before adding 10 mL of HCl that had been measured in a graduated cylinder beforehand. The stopper was quickly and firmly placed into the test tube and the pinch clamp was removed again. After a few minutes the acid dissolved the capsule creating a black foam that then turned into a clear liquid again.
After the mixture was heated, there was solid on the bottom and liquid on the top of the flask. The set up was let cool to room temperature. Next, we decanted the mixture in to a clean 100 ml beaker. We rinsed the remaining solid with 10 ml of DCM and swirled the mixture. The mixture was decanted again in to the same beaker.