The unknown bacteria was taken from the NA agar slant and placed on an oxidase strip in a drop of distilled water. Within seconds the bacteria will turn purple if oxidized cytochrome oxidase is present, indicating a positive test. If no color change is observed, no cytochrome oxidase is present and the test is negative. My results for oxidase test were positive. To conduct the oxidase test I first obtained an oxidase strip.
Methyl Red turns red for a PH that is lower than 4.4 and it turns orange for a Ph between 4.4 and 6.0. Methyl Red will turn yellow with a Ph of 6.0 or above. Red means that the bacteria produces stable acid end products, yellow color means no acid end products, and orange is inconclusive. ("Lab manual," nd, p. 140) the Voges-Proskauer test determines the
Protects the bacteria from phagocytosis allowing the bacteria to stay in the body 6. pure culture 7. It is differential based on hemolysis of the agar. Hemolysis can be wide-narrow band beta, alpha, gamma, or none. 8. candle jar in microbiology is used for anaerobiosis in which a lit candle is placed in an air tight jar and if it went out, it would be because it used up all the available oxygen. 9. any streptococcus capable of hemolyzing erythrocytes, classified as α-hemolytic type, producing a zone of greenish discoloration much smaller than the clear zone produced by
Abstract The purpose of the experiment was to identify unknown ionic compound #. After many tests, the unknown was identified as sodium chloride. The cation (Na+) was determined by having a yellow/orange color flame test. The anion (Cl-) was determined by the chloride anion test when the unknown test solution showed a positive test for chloride. The synthesis of NaCl further identified the ionic compound by reacting sodium hydroxide and hydrochloric acid and obtaining solid sodium chloride.
BIOL Experiment 2 - Lab Report Characterization of Phosphatase Activity in a Crude Alfalfa Sprout Extract Name: tereasa avalon Circle your lab section: T–AM | W-AM | R-AM | F-AM | T–PM | W-PM | R-PM | F-PM | Instructor: Dr. kaiba Graduate TA: Tristan Date: 9/19/2012 Partner: joey wheeler 1. (6pts) Construct two separate bar graphs depicting your results for the effect of pH and temperature on phosphatase activity. All figures/graphs/tables must include appropriate titles, legends, labeled/ scaled axes, and standard deviation bars. Do not include a figure caption. Attached to the back 2.
6. Wash, dry and observe under oil immersion lens. Differential stains render one type of microbe one colour and other types of microbes another colour. In the Gram stain, Gram positive organisms retain the primary dye complex (crystal violet-iodine) whereas Gram negative cells loose the primary dye complex during the challenge rinse. Most differential stains have a challenge step that follows staining with a primary dye.
Ethan D. Wilhelm September 10, 2012 Chemistry 121 section 8 Friday 9-12 Sarah Gains Unknown Substance Lab Purpose: The purpose of this lab was to identify an unknown substance B by finding enough of its physical properties that we could eliminate all substances it can’t be. We use micro lab to help us find the boiling point, freezing point and mass. Then we tested the solubility of the unknown substance in water, hexane, and Alcohol. With this information we were able to find the identity of the unknown substance B. Procedure: Throughout this lab we were finding the physical properties of substance B.
For the food test that use to determine the of presence protein we use Biuret test instead of Million’s Test. The reason why we are choosing the Biuret test is because the Millon’s Test cannot have positive result on specific protein such as gelatine due to the gelatin had no tyrosine beside the Million’s Test will show positive result on every substance which had the tyrosine including non protein substance. So we used albumin solution and it will only show positive result on protein only. Objective -To determine the presence of reducing sugar by carrying of Benedict’s test -To determine the presence of non reducing sugar by carrying out Hydrolysis process by using dilute hydrochloric acid. -To show the presence of starch by carrying out iodine test.
Enzymes Amylase: | Tracing the Breakdown of Starch | Rashaud Pickering, Ashley Bagnis, Stephanie Alvarado, Elbany Angulo 3614098 Section U-14 | Signature___________________ Abstract An experiment was conducted to find out what the optimal temperature for amylase to break down starch was. An Iodine test was used to find evidence of starch formation. Three drops of iodine were dropped into two sets of spot plates. There were four test tubes that contained human amylase and four test tubes that contained fungal amylase. These specimens were all tested before being placed into their respective ice or water baths.
What did those mangy tagi do? What are you going to do to relieve this headache? How will you survive……. Purification of Organic Compounds Thank goodness that you are in organic chemistry lab and not in the South China Sea. One of the important aspects of organic chemistry is being able to take an impure mixture of organic molecules, identify it as a mixture and purify each component.