The unknown bacteria was taken from the NA agar slant and placed on an oxidase strip in a drop of distilled water. Within seconds the bacteria will turn purple if oxidized cytochrome oxidase is present, indicating a positive test. If no color change is observed, no cytochrome oxidase is present and the test is negative. My results for oxidase test were positive. To conduct the oxidase test I first obtained an oxidase strip.
The purpose of these tests were to determine how the bacterium reacted to glucose with and without oxygen. My findings reported a gram stain negative along with positive tests for glucose oxidation and fermentation. Next I performed an Indole Production Test. This test determines whether the microbe produces indole from the amino acid tryptophan. The results from this test were negative.
Effect of ammonium nitrate on Ceratopteris richardii Abstract: Ceratopteris richardii, also known as C-fern, is commonly used in research studies as a model plant because of its short generation time. In this experiment, we aimed to observe the effects of ammonium nitrate on this species of ferns. To achieve this, we inoculated and evenly spread three drops of C. richardii spores into two separate and sterile petri dishes, one control group and one experimental group. The experimental treatment contained 1% ammonium nitrate on the medium and the control treatment was merely a normal nutrient-rich agar. Then, we performed an f-test and a t-test to determine if there was any germination difference between the treatments.
Introduction In Project 2, the goal of the experiment was to test for the active acidic or basic ingredients in a commercial product. By using standardized solutions of HCl and NaOH to perform a titration of the chosen commercial product, which was Vitamin C tablets and lime juice, the ascorbic acid content could be taken and compared to the manufactures standards. The information that was gained in this experiment can be compared to the manufacture’s stated amount of Vitamin C and lime juice in the nutrition facts, and our rests would either refute their measurements or prove them to be truthful. If our tests concluded that the sample of their product was so far off that it exceeds a 20% error, it will be out of the FDA standards of acceptable error and in turn be considered defective. Methods My group used the method of titration to test whether the Vitamin C and lime juice nutritional facts where correct from their manufacture.
Courtney Sever Period 2 10/11/12 Experiment 3: Beer’s Law Purpose/Hypothesis: The reason we prefer to express the law with this equation is because absorption if directly proportional to the other parameters as long as the law is obeyed. In this experiment we are going to test different solutions and then test an unknown. Materials: CBL Colorimeter Test tubes (6( Test tube rack 8 Cuvettes Pipets Gatorade (unknown) Blue Stock solution Water Beakers Paper Towels Procedures: * Turn on the CBL, and open the apps to the Colorimeter setting. * Fill one cuvette with water, making sure the outside is completely dry, and set in the colorimeter to calibrate it. * Then label each test tube, 1-6.
Dr. Følling was one of the first physicians to apply detailed chemical analysis to the study of disease (Phenylketonuria, n.d.). His careful analysis of the urine of two affected siblings led him to request many physicians near Oslo to test the urine of other affected patients (Phenylketonuria, n.d.). This led to the discovery of the same substance that he had found in eight other patients (Phenylketonuria, n.d.). The substance found was subjected to much more basic and rudimentary chemical analysis (taste) (Phenylketonuria, n.d.). He conducted tests and found reactions that gave rise to benzaldehyde and benzoic acid, which led him to conclude the compound contained a benzene ring (Phenylketonuria, n.d.).
Name ___________________________ Chapter 23 • Functional Groups Date ___________________ Class __________________ SMALL-SCALE EXPERIMENT 38 VITAMIN C IN TABLETS Small-Scale Experiment for text Section 23.3 OBJECTIVES • Determine the vitamin C content of vitamin C tablets by iodometric titration. • Observe the oxidation of vitamin C in aqueous solution. INTRODUCTION Vitamins regulate biochemical reactions that take place within living cells. The human body requires vitamins only in tiny amounts. For a compound to be classified as a vitamin, its absence in the diet must cause a specific disease that is cured when the vitamin is resupplied.
For those that tested negative, 15 M NH4OH was added in the ammonium hydroxide test to observe for the precipitate color which further separated the cations. The final step was the flame tests which confirmed the cations’ identity. After a Bunsen burner was setup, a Nichrome loop was dipped in HCl and placed above the flame until no color was observed in order to more accurately observe the color of the cation when placed in the flame. Each cation was added into centrifuge
Tetracycline. Novobiacin. Because Vibrio Fischeri is a bacteria usually underwater and is only sensitive to aquaculture antibiotics, our antibiotics were not successful. None of the antibiotics used were meant for ridding of underwater bacteria. The home cleaning agents were more successful, especially the dish soap.
Saint Isidro’s Bilingual School Guillermo Carrillo Biology 11th Grade Spontaneous Generation Needham’s Experiment John Needham challenged Redi's findings by conducting an experiment in which he placed a broth, or “gravy,” into a bottle, heated the bottle to kill anything inside, and then sealed it. Days later, he reported the presence of life in the broth and announced that life had been created from nonlife. In actuality, he did not heat it long enough to kill all the microbes. Spallanzani’s Experiment He reviewed both Redi's and Needham's data and experimental design and concluded that perhaps Needham's heating of the bottle did not kill everything inside. He constructed his own experiment by placing broth in each of two separate bottles, boiling the broth in both bottles, then sealing one bottle and leaving the other open.