Unknown Microorganism

1833 Words8 Pages
Abstract Isolation plays a key part in the identification of unknown microorganisms. To be able to successfully identify an unknown microorganism, it is necessary to have single, isolated colonies as references for the tests. Throughout this report, we will be examining the differences in unknown bacteria types, whether it is Gram Positive or Gram negative and will be looking at ways to distinguish the difference between the various unknown bacteria samples that are going to be used. The ultimate aim of this experiment is to correctly identify the different types of unknown microorganisms provided to us and differentiate their types. To do this, we will be using 8 different widely know techniques, including the use of streak plate, gram…show more content…
It is for this reason that Identification of bacteria is very important in the many areas of microbiology (Singh, 2009). There are many reasons for identifying an unknown bacterium. The reasons range from medical purposes, such as determining if the unknown could cause ailments in living things or knowing what microorganisms are needed to make antibiotics to other purposes such as knowing the exact microorganism has to be used to make certain foods (Burton, 2003). Throughout this report we will be addressing the differences in methods of identifying unknown microorganisms and differentiate between the results of these techniques by explaining how each outcome was achieved. The main aim of these experiments is to determine whether the unknown microorganisms are of Gram-Positive or Gram Negative nature and ultimately to determine exactly what kind on microorganism the unknowns are. During this experiment we will be working with 6 different…show more content…
Eosin methylene blue and Mannitol agar plates will also be used to determine pH values. Test Three: Gram Stain – A basic technique which will help to distinguish between a Gram-positive and Gram-negative bacterium. Test Four: Catalase assay – Is to be used to determine whether the unknown bacteria will liberate free O₂ gas. Test Five: Oxidase assay – The cytochrome Oxidase will aid in determining whether the unknown bacteria has an absence of Oxidase or not. Test Six: Glucose assay – Whether an organism can respire or ferment glucose can be tested with this Glucose O/F medium assay. Test Seven: Oxidation Fermentation assay (O.F.) – “The original intention of this medium was to be able to differentiate between Gram-negative bacteria that can: - Ferment - Only catabolise glucose by respiration - Do not catabolise glucose at all” (Singh, 2009). Test Eight: Motility assay –This technique will also be used to determine whether the unknown bacterium is Gram-positive or

More about Unknown Microorganism

Open Document