Pseudomonas Aeruginosa The purpose of this lab was to identify the unknown bacteria culture given by our respected instructor using various differential tests. The identification of this unknown culture was accomplished by separating and differentiating between possible bacteria based on specific biochemical characteristics. Each test was used in a way to help recognize those specifics and bring us one step closer to identify the unknown cultures. The differential tests used to identify the unknown cultures were oxidase, lactose and glucose fermentation, citrate, indole, urease, motility, H2S, EMB and MAC. The tests performed on the unknown bacteria culture were all used to determine and identify the bacteria.
What kinds of clinical specimens may yield a mixed flora in bacterial cultures? Oral, Skin, or GI specimens 5. When more than one colony type appears in pure culture, what are the most likely sources of extraneous contamination? Individual colonies can be picked up on the inoculating loop, or straight wire and inoculated in to the fresh agar or brother media References Cowan, M. K. (2012). MICROBIOLOGY: A SYSTEMS APPROACH, THIRD EDITION.
We were dealing with bacteria that has the ability to be harmful and to avoid illness/sickness we need to thoroughly wash our hands and be careful around the bacteria. Another precaution to take was to not cough into or put any bacteria from our bodies into the agar plate. Those bacteria could be extremely dangerous and shouldn’t be cultivated. Observations Located on final page(s) Application Questions 1. The purpose of heating the agar plates in the autoclave prior to inoculating them with microorganisms was to make sure that the lab results were exact.
In order to find out which antibiotics work on the bacteria, doing an agar plate test is the best way to get answers. Results; I tested the affect of a series of antibiotics on two ranges of cultures. E.Coli and S.Albus. I used four agar plates. Two for E.Coli and two for S.Albus.
Exploring enzymes through Lactex. Problem: Does pH affect the results of Lactex? Independent Variable: pH value Dependent Variable: Glucose Control: Lactex enzyme Hypothesis: I think pH will cause the result of Lactex to change. Materials: · Soap (basic) · Antacid (basic) · Soda (acidic) · Vinegar (acidic) · pH test strips · eye dropper · Cow’s milk Experimental Design: 1. Set up plastic cups 2.
For the food test that use to determine the of presence protein we use Biuret test instead of Million’s Test. The reason why we are choosing the Biuret test is because the Millon’s Test cannot have positive result on specific protein such as gelatine due to the gelatin had no tyrosine beside the Million’s Test will show positive result on every substance which had the tyrosine including non protein substance. So we used albumin solution and it will only show positive result on protein only. Objective -To determine the presence of reducing sugar by carrying of Benedict’s test -To determine the presence of non reducing sugar by carrying out Hydrolysis process by using dilute hydrochloric acid. -To show the presence of starch by carrying out iodine test.
5) Botulism can also be contracted through an open wound. This type of botulism is wound botulism and it is one of the three main types of botulism. In food-borne botulism: Types of home-canned foods provide a perfect condition for the bacteria to grow. If botulism is not treated, it will eventually lead to respiratory problems, paralysis and death. If diagnosed early, botulism can be treated with an antitoxin.
Method: In this investigation same volume of 3 different types of orange juice are used, to find out which one has the most Vitamin C. This investigation involves using the organic indicator called Dichlorophenolindophenol which abbreviates to DCPIP. The indicator is a dark blue colour which turns colourless when a known amount of it is used neutralize ascorbic acid which is Vitamin C. The amount of juices used to turn the DCPIP colourless will be recorded and the vitamin C content will be determined by measuring the amount of a known vitamin C concentration
Biology lab report ; topic : 3.2 ____________________________________________________________ ______________________ Title : Estimating Glucose Concentration in Solution Aim : To estimate the glocose concentration in several solutions which have the same volume. Introduction : In this experiment, we have to estimate glucose concentration in a particular solution. The nature of a glucose is a monosaccharide reducing sugar. Since glucose will be oxidised, it acts as a reducing agent which will donates electrons that will be accepted by the purple pink potassium permanganate. Thus, the pink purple colour of potassium permanganate will be reduced into manganese ions.
| Practical Report | | | | Practical Report Introduction This specific experiment will demonstrate both the efficiency and the disinfecting capabilities of the various disinfectants used and if the experiment is followed correctly the results deemed should be noted that if the substance possess a chemical substances that inhibits the growth of the bacterial culture placed onto the agar (E.Coli) that inhibition zones will be created and we can tabulate and record the observation thus walking away with an understanding of the efficiency of the tested disinfectants used and their success rate. What is known about E.Coli is that it is normally found in food it is a Gram negative, rod shaped bacterium that can help the body break down and assists the body in digestion of food (Dowshen, 2009).Although certain strains of the E.Coli are pathogenic to humans such as the serotype O157:H7, this specific strain of E.Coli is known to cause food poising and can escalate and become life threatening (Nordqvist, 2011). Domestos is chlorine based bleaching agent (Directa, 2009) and is a common household cleaning substance that is used. Handy Andy is a common and popular general house hold cleaner. Dettol is trusted antiseptic and disinfectant and is widely used to fight unwanted bacteria (Thompson D. M.) It has a distinct and characteristic phenolic odour.