6) The ether was removed using the rotary evaporator. We discarded the used MGSO4 into solid waste containers. The solid that remained after ether evaporated was the neutral organic compound. 7) We scraped the solid from the flask with a spatula and allowed the solid to dry thoroughly on a piece of filter
EXPERIMENT ( 3 ) Topic : The preparation of Phenyl Benzoate from benzoyl chloride Introduction : The preparation of phenyl benzoate (ester) from phenol and benzoyl chloride is an esterification. In this experiment, you are requested to prepare and purify phenyl benzoate by some techniques. Chemicals : Phenol, Benzoyl chloride, Dilute NaOH, Methanol Procedures : 1. Dissolve 1 g phenol in 15 cm3 dilute NaOH using a 50 cm3 quickfit flask. 2.
Cylinder was rinsed with distilled water. * * 2. 2.0 ppm standard: 2.00 mL of 10.0 ppm phosphate solution was placed in a 25 mL graduated cylinder and diluted to exactly the 10 mL mark with distilled water then poured into a plastic cup labeled 2. Cylinder was rinsed with distilled water. * 3.
Then 5mL of HCl was added to copper to completely remove all traces of zinc. Once the bubbling had stopped, the rest of the liquid was decanted away from the copper. Then the copper recovery set up was put together using tubing, Buchner funnel, filter paper and suction flask. Then the filter paper was weighed before placing it in the funnel and wetted down. The aspirator was turned to medium high, and then the copper was poured onto wetted filter paper.
Add a quarter spatula of copper (II) oxide and warm the solution gently to the 4th test tube and record observations. 7. To the 5th test tube, add 3cm3 of ethanol a couple of drops of conc, sulphuric acid and warm gently. Pour the resulting mixture into 30cm3 of sodium carbonate solution to remove excess acid and smell and record observations. Experiment 2 Time | Observations | 5 minutes | Bubbled like sugar | Once salt water was added | Turned soapy white and thick | Equation: METHOD 1) Put 2 cm3 of castor oil into a 250 cm3 beaker and add 10 cm3 of 5mol.dm-3 sodium hydroxide from a measuring cylinder.
Discussion & Conclusion In this experiment we learned how to synthesize the cyclohexene by dehydration of cyclohexanol. We procedure the first step where we have to mix the components. Then we heat the R.B.F with a fractionating column, distilled water. Then we obtained the layers, and we transferred the organic layer to a small, dry Erlenmeyer flask. We added anhydrous Sodium Sulfate as a drying agent.
After a series of washes to remove any impurities, the compound was dried and decanted, cholesterol was synthesized. Melting points were obtained from commercial cholesterol, dibromocholesterol, and synthesized cholesterol using the Fisher-John Melting Point Apparatus. A TLC plate was completed, spotting commercial cholesterol, dibromocholesterol, and synthesized cholesterol. The silica gel was used as the stationary phase and the ethyl acetate: hexane (30:70) was used as the mobile phase. The silver nitrate test was conducted to test for the functional group of 2°and 3° alkyl halides as positive and negative controls were used.
Transfer the distillate via Pasteur pipet into a 15-mL centrifuge tube. Wash the distillate with two 2.5-mL portions of water, removing and setting aside the water layer at each step. Transfer the organic material to a small, dry Erlenmeyer flask, leaving behind the last drops of the water layer. Remove any drops of water that were accidentally transferred to the Erlenmeyer flask, if any, then add small amounts of anhydrous sodium sulfate as drying reagent. When the liquid is dry, it will be transparent and some of the drying agent will flow freely like beach sand.
Add a drop of water using and inoculating loop to a clean glass slide. Then using a sterilized loop, smear the slide with the organism, let the slide dry completely and heat fix. Apply crystal violet stain (primary stain) for one minute, briefly rinse with distilled water. Apply iodine (mordant) for one 1 minute, briefly rinse with distilled water. Then proceed to decolorize with 95% ethanol until the ethanol running off the slide is clear.
The mechanism for the reduction of a ketone by sodium borohydride: (1) Method (Based on Chemistry Laboratory Manual): 1. 0.4510g of benzophenone was dissolved in 5 cm3 of ethanol in boiling tube containing a magnetic stirrer bar. 2. O.1249g of sodium borohydride was added and left for 30 minutes. 3.