Cholesterol Essay

2553 Words11 Pages
Samantha Monette Lab Section E Wednesday 10:00-12:50 p.m. Bromination/Debromination of Cholesterol Abstract: The purpose of this experiment was to purify commercial cholesterol. The commercial cholesterol was brominated using bromine as the reagent. After the compound was cooled and vacuum filtrated, dibromocholesterol was obtained. To purify the dibromocholesterol, zinc was added as the reagent. After a series of washes to remove any impurities, the compound was dried and decanted, cholesterol was synthesized. Melting points were obtained from commercial cholesterol, dibromocholesterol, and synthesized cholesterol using the Fisher-John Melting Point Apparatus. A TLC plate was completed, spotting commercial cholesterol, dibromocholesterol, and synthesized cholesterol. The silica gel was used as the stationary phase and the ethyl acetate: hexane (30:70) was used as the mobile phase. The silver nitrate test was conducted to test for the functional group of 2°and 3° alkyl halides as positive and negative controls were used. The silver nitrate test was conducted for a presence of the functional group alkenes and there were positive and negative control. The percent yield of dibromocholesterol collected was 91.98% and the percent yield of synthesized cholesterol collected was 140%. The percent recovery of the commercial cholesterol was 127%. This can only suggest there were some impurities in the synthesized cholesterol but other results prove it to be pure. The TLC plate proved the synthesized cholesterol to be pure because there were only the three spots and the commercial cholesterol and synthesized cholesterol were relatively close to each other. UV light and an iodine tank was used for detection. The melting points also proved the synthesized compound to be pure because the range was close to the commercial cholesterol. The dibromocholesterol yielded a positive

More about Cholesterol Essay

Open Document