Eosin methylene blue and Mannitol agar plates will also be used to determine pH values. Test Three: Gram Stain – A basic technique which will help to distinguish between a Gram-positive and Gram-negative bacterium. Test Four: Catalase assay – Is to be used to determine whether the unknown bacteria will liberate free O₂ gas. Test Five: Oxidase assay – The cytochrome Oxidase will aid in determining whether the unknown bacteria has an absence of Oxidase or not. Test Six: Glucose assay – Whether an organism can respire or ferment glucose can be tested with this Glucose O/F medium assay.
3. Why is it necessary to make pure subcultures of organisms grown from clinical specimens? So that the organism can be identified and tested for antibiotic sensitivities. 4. What kinds of clinical specimens may yield a mixed flora in bacterial cultures?
Examine your living organism and determine if it is a bacteria, achaean or a eukaryote. At each step in classification, check the requirements for each category to determine where the species belongs. You then would begin to ask yourself a series of questions about the organism one question will lead to another. If the organism cannot be identified through the questions you will than need to do a gram staining process. You will look at the bacteria through a microscope exposing the bacteria's cell wall to two types of stains: a violet and a red one.
As a more polar solvent is use to push the different rings of pigment, these are collected in their own test tubes to then be run in a TLC which will determine the polarity using the Rf values and then comparing them to the table in the organic lab manual ones. This is important because we want to know what some things are composed of and by using the polarity in different components makes it easier to determine them. The gain from this experiment is determining the different pigment of spinach and by doing so comparing the polarity of each of them. Then determine why that could be by looking at the structures of the pigments separated. Discussion: The first thing done in
Hypothesis: If bacteria with + pGLO plasmids that are resistant to the antibiotic ampicillin and have the gene for GFP, colonies with survive and grow on the transformation plates that have LB/ amp. In addition, + pGLO bacteria on a plate with LB/amp/ara will grow and glow green under UV light because of the inclusion of arabinose. In the control plates, -pGLO bacteria that are amp sensitive will not be able to grow on the LB/ amp plates. The other control plates with – pGLO bacteria and no ampicillin added will host a lawn of colonies. IV.
The streak plate technique is done to isolate a colony formed by a single cell from a mixture containing millions of cells. The streak plate technique was also used to obtain pure cultures of the bacteria using a trypticase soy agar (TSA) plate and was incubated at 37 ̊ for 48 hours. Secondly a Gram stain was performed as directed from exercise 6in the lab manual (Kleyn 37) and was found to be Gram negative Rods. The Gram stain is important to do because it reveals the morphology of the organism and the arrangement of the cells; if an organism appears purple under a microscope then it is said to be Gram positive, and if it
13. State two observable characteristics that you can use to distinguish an animal cell from a plant cell based on what you saw using the compound light microscope. Show your work. Record the high-power field diameter both in millimetres and micrometres in your table. Skill Practice Part 2 — Estimating Cell
Lab Report: “The Unknown” NAME: Donnise Warren PURPOSE: The purpose of this lab report is for students to be able to identify an unknown bacterium given to us by doing a variety of lab procedures and biochemical lab test to help identify what specific bacteria we have. INTRODUCTION: There are many reasons for knowing the identify of microorganisms. Such as knowing the causative agent of a disease that a patient may acquire, so that you would know how it could be treated, to knowing which microorganisms could be used for making antibiotics or even food. This lab report was done by implementing methods that have been learned throughout this semester in our microbiology lab course which helped identify my unknown bacterium #115. 6/28/2012 The 1st lab procedure performed was the gram stain.
After examining the isolated unknown organism using the 40X objective with the phase contrast filter, I observed that the cells appear to be rod shaped and non-motile. After examining my soil sample under phase microscopy, I performed gram staining in order to determine whether my bacteria are of the class proteobacteria or firmicutes. I gram stained S. epidermidis
September17.2013 P.4 Environmental Sources Of Bacteria Purpose: We did this lab to find out exactly which conditions would help to grow a bacterium the best. Materials: • Agar • Petri dish • Cotton • Incubator • Tape • Bacteria Procedure: 1. Mark bottom of Petri dish 2. Split petri dish into 4 sections and label the sections 3. Pour hot agar into dish 4.