Ap Biology Transformation Lab

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I. Title: pGLO Transformation Lab II. Purpose; We want to be able to observe, comprehend and analyze genetic transformation as we genetically alter organisms. III. Hypothesis: If bacteria with + pGLO plasmids that are resistant to the antibiotic ampicillin and have the gene for GFP, colonies with survive and grow on the transformation plates that have LB/ amp. In addition, + pGLO bacteria on a plate with LB/amp/ara will grow and glow green under UV light because of the inclusion of arabinose. In the control plates, -pGLO bacteria that are amp sensitive will not be able to grow on the LB/ amp plates. The other control plates with – pGLO bacteria and no ampicillin added will host a lawn of colonies. IV. Materials V. Procedure VI. Observations/ Data; Lesson Three Collection and Analysis: A) Data Collection; 1. For the first plate, +pGLO (LB/amp), there was not a lot of growth on the plate, in comparison to the other groups and it did not glow under UV light. For the second plate, +pGLO (LB/amp/ara), there were even fewer colonies observed, unlike any of the other groups as well, and it did glow under UV light. For the third plate, -pGLO (LB/amp), there was no growth at all, much like every other groups. Finally, the fourth plate –pGLO (LB), displayed a lawn of growth, much like other groups plates as well, and did not glow under UV light. 2. The first plate had two colonies both larger spots that were across the plate from each other growing on the sides or ledges of the plate. The second dish also had either one large colony, or two smaller ones that had grown together fusing to create one large colony, on the ledge of the plate. The third plate did not have any growth. The forth plates exhibited too many colonies to count; there was a lawn of growth, a fuzzy white covering of bacteria that stretched across

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