2. What morphological structure is responsible for bacterial motility? Flagella 3. Why is a wet preparation discarded in disinfectant solution or biohazard container? Because of the Preparation of micro- organisms which can interact with the people or environment surrounding them.
Differential Staining Brenda Blanchette, RN, CCTC Clinical Microbiology Laboratory Differential Staining There are several staining methods used with bacteria and are generally classified as simple, nonspecific, or differential (specific). Gram’s stain techniques are used to differentiate between types of bacteria as well as to differentiate between Gram-positive and Gram-negative bacteria. Gram’s stains are one of the most commonly used differential stains and are the focus of this experiment (Labpaq microbiology). Bacteria that stain purple are Gram-positive and bacteria that stain pink are Gram-negative. They stain differently due to differences in the structure of their cell walls.
In comparison of the descriptive chart and the comparative analysis sheet, 18 out 18 of the tests performed were alike. This data helped me identify this unknown bacteria and understand why it was that bacteria. Enterobacter aerogenes is a facultative anaerobic bacteria that is present in the intestinal tract of humans. This bacteria naturally occurs in soil and freshwater and is present on plants, sewage, and is an opportunistic pathogen present on human skin. Antibiotics can be used to treat the
It is for this reason that Identification of bacteria is very important in the many areas of microbiology (Singh, 2009). There are many reasons for identifying an unknown bacterium. The reasons range from medical purposes, such as determining if the unknown could cause ailments in living things or knowing what microorganisms are needed to make antibiotics to other purposes such as knowing the exact microorganism has to be used to make certain foods (Burton, 2003). Throughout this report we will be addressing the differences in methods of identifying unknown microorganisms and differentiate between the results of these techniques by explaining how each outcome was achieved. The main aim of these experiments is to determine whether the unknown microorganisms are of Gram-Positive or Gram Negative nature and ultimately to determine exactly what kind on microorganism the unknowns are.
The cell wall of the bacteria 3. What is iodine used for in this staining technique? A. Iodine is used to affix the primary stain crystal violet to make a large molecule that will not be easy to be washed away. 4. Why is it important to heat fix
However, when the dead skin from the epidermis occasionally blocks a sebaceous gland of a hair follicle, the bacteria may infect surrounding tissue resulting in inflammation and causing a pimple. 4. a) What is the pH of a substance? • In chemistry, pH (Potential Hydrogen) is a measure of the acidity of an aqueous solution. b) How does the pH describe the acidity of a substance? • It uses a scale to describe the acidity level and the pH scale ranges from 0 to 14.
Examine your living organism and determine if it is a bacteria, achaean or a eukaryote. At each step in classification, check the requirements for each category to determine where the species belongs. You then would begin to ask yourself a series of questions about the organism one question will lead to another. If the organism cannot be identified through the questions you will than need to do a gram staining process. You will look at the bacteria through a microscope exposing the bacteria's cell wall to two types of stains: a violet and a red one.
Fractions 1,2, and 3 were all separated by column/flash chromatography and their purities were measured with the TLC plate. One suggestion for future labs would be to use more accurate measuring tools. Post lab 1. So the solvent would not reflect the UV rays, therefore creating a dark spot on the luminescent TLC plate. This dark spot helps us measure how much the solvent moved on the TLC plate.
This was confirmed by the lab manual in page 30 which contains the list of the different Rf values by decreasing value. Introduction: Chemist use many ways of separating and determine want components are in different materials. One of this ways is by dissolving the material in solvent this will only get the mixture of components for example the spinach had to be grind up with hexane to get the organic material separated from the pigments that will be separated even further. Next using a solvent and a polar compound like alumina, the pigments separated from the organic material could be run in a column in which those will be separated even more using polarity. As a more polar solvent is use to push the different rings of pigment, these are collected in their own test tubes to then be run in a TLC which will determine the polarity using the Rf values and then comparing them to the table in the organic lab manual ones.
Which contaminant seemed to have the most potent effect on the water? The effects of the contaminated water were that the mixture showed each water type to have a brown color to them. The water type that seemed to have both a surprising and potent effect was the water and soil mixture. I would have thought with using strictly water and potting soil the water turned the darkest brown out of all the other contaminants that I used for the mixture. The vinegar and soil mixture seemed to have cleaner look to them.