Next, take the cotton swab and drag it lightly in a zigzag motion across the agar on the petri dish; this is called streaking. Create two sections in each of the petri dishes by drawing a line down the middle of the outside plastic with a permanent marker. Then, place the four bacterial inhibitors disks in the center of the four sections of the petri dish. Make sure to soak each disk in the bacterial inhibitor for twenty-four hours. Lastly, put the lids back on the petri dishes and put in the incubator at 37 degrees Celsius for 24 hours.
Bacteria Lab AP Biology Bacteria Lab over School Sinks Purpose/Problem Statement: The main purpose of the “Bacteria Lab Over School Sink” was to learn more about bacteria, where is it found, how cleaning can affect the amount of bacteria, and compare data samples between boys and girls hygiene. Background: According to Biology Online bacterium or bacteria is a microscopic, single celled organism(s) which belong to a kingdom named Monera. These organisms have a prokaryotic cell structure. When an organism is prokaryotic it means that the cells lack membrane-bound organelles. Bacteria are highly successful when reproducing and adapting due to their reproductive time (1-pg.
In determining this unknown bacteria, number two, many tests were performed. The tests that helped identify this bacteria were gram-stain, motility, Simmons citrate, lactose testing, and methyl red testing. All of the tests that were stated and recorded on the comparative analysis chart also tribute to uncovering the unknown bacteria, but they were not the critical tests needed. In the gram-stain tests, the bacteria was indicated as negative and the shape of the bacteria were rods. Motility was noted under the microscope by evidence of the bacteria moving around on the slide.
E. coli has even been used to synthesize human insulin. Other strains are pathogenic, causing diarrhea, urinary tract infection and even fatal illness. Unlike some bacteria, E. coli cannot tolerate harsh environments, and instead thrive at 37°C. The primary goal of this experiment is to examine the growth of E. coli on bacterial lawns in order to determine expired mouthwash’s efficiency as an antibacterial product. Since it is expired, the mouthwash should not perform well as an antibacterial agent.
How Does Temperature Affect the Growth of Mold on Wheat Bread? | Researched by Nichole A. 1999-2000 | * PURPOSE * HYPOTHESIS * EXPERIMENT DESIGN * MATERIALS * PROCEDURES * RESULTS * CONCLUSION * RESEARCH REPORT * BIBLIOGRAPHY * ABOUT THE AUTHOR | PURPOSEThe purpose of my experiment was to determine how temperature would affect the growth of mold on honey potato bread. The reason I became interested in my topic was because I thought it would be helpful to find a way to keep food from molding. The information gathered in this experiment would help scientists who study mold and people who hate mold on their food.
Methods which have been used throughout the semester to identify specific bacteria were applied to the unknown. The methods and procedures were followed as the laboratory manual for microbiology stated, unless stated otherwise. On day one, the first objective was to complete an isolation streak of the mixed culture onto nutrient agar. The goal of this test was to correctly isolate the two different bacteria. The nutrient agar was incubated at 37 degrees Celsius for 48 hours.
The home cleaning agents were more successful, especially the dish soap. Shout had some growth in the end, but not nearly as much as any of the antibiotics. Dish soap is used in water to clean dishes and it seemed that because it was used in water it was most successful against the Vibrio Fischeri. Antibiotics are selective, and none of the ones used in this experiment were meant to be used against Vibrio Fischeri. This is why none of them worked to prevent the bacteria from spreading.
Getting these pedicures or manicures can also put you at risk for developing skin allergies, either from the glues or some of the chemicals. Not only can people develop diseases through the chemicals, some customers have become infected with nail fungus from dirty tools used in the nail salons. This is a communicable disease; another related one is the ringworm. Both the drills which are used sometimes and cuticle pushing can damage the matrix of the nail, causing permanent loss or deformity. Another area of concern while getting your pedicure or manicure is usage of these recliner chairs with their built in foot baths.
The Relationship between Different Antibiotics, Vancomycin and Chloramphenicol with the bacteria,Staphylococcus aureus Cooper, Dalton and Alison Kim HC59 BOX1422 Cabins, WV 26855 Abstract During this experiment we observed which antibiotic, Vancomycin or Chloramphenicol will be most effective in controlling the growth of Staphylococcus aureus. The hypothesis that we tested was that both Vancomycin and Chloramphenicol will be effective in controlling the growth of S. aureus bacteria. Next we placed a disc in each disc, one containing Vancomycin, one containing Chloramphenicol, and one containing nothing in their respective dishes which we replicated eight times. We waited twenty-four hours for the cultures to develop and then refrigerated them for seventy-two more hours until we could collect the results. The mean death zone was +/- one standard deviation for the control was 0 +/- 0mm, for Chloramphenicol it was 26.25 +/- 1.39mm, and for Vancomycin it was 19.125 +/- .83mm.
The slide was flooded with malachite green before passing over to the flame several times until it “steamed”. The stain was replenished as it was continued to pass over the flame without letting it to boil. After it was rinsed with tap water, safranin was added as a counterstain. It was rinsed and air-dried before it was examined under OIO. B. Bacterial Capsules A small drop of nigrosin was placed near the edge of a clean slide.