Lab Report: “The Unknown” NAME: Donnise Warren PURPOSE: The purpose of this lab report is for students to be able to identify an unknown bacterium given to us by doing a variety of lab procedures and biochemical lab test to help identify what specific bacteria we have. INTRODUCTION: There are many reasons for knowing the identify of microorganisms. Such as knowing the causative agent of a disease that a patient may acquire, so that you would know how it could be treated, to knowing which microorganisms could be used for making antibiotics or even food. This lab report was done by implementing methods that have been learned throughout this semester in our microbiology lab course which helped identify my unknown bacterium #115. 6/28/2012 The 1st lab procedure performed was the gram stain.
Unknown Lab Report: Enterobacter cloacae Jessica Sainvil Professor Cupido Bio 253L1 Thu 9:30-12:30 Due Date: 12/6/12 Unknown#103 Introduction The bacterium Enterobacter cloacae isolated from the given #103 was a Gram-negative Rod. The purpose of this lab was to isolate and identify the genus and species of an unknown bacterium. It is important to identify an unknown microorganism because knowing how the bacteria work and how it is structured means knowing how it can affect humans. Unknown bacteria may also be used clinically many pharmaceutical drugs are based on products made by organisms (Katzung, B.G.2004). In order to identify the unknown organism a series of tests were performed.
In order to find out which antibiotics work on the bacteria, doing an agar plate test is the best way to get answers. Results; I tested the affect of a series of antibiotics on two ranges of cultures. E.Coli and S.Albus. I used four agar plates. Two for E.Coli and two for S.Albus.
The purpose of this lab is to focus on how to make zinc iodide in a different way using compounds instead of elements, which are barium iodide and zinc sulfate. We will see if the reaction between these two compounds will occur and make a prediction by writing a chemical equation. The procedures for this lab are to place a small test tube inside a 50mL beaker and weigh it. Then, using a spatula, add 0.45±0.03 g of zinc sulfate heptahydrate into the small test tube and record the mass. After that, dissolve the sample in 2 mL of deionized water and shake the test tube for 1 to 1 ½ minutes to dissolve the solid.
pBlu Bacterial Transformation Purpose: To change the DNA structure of bacteria to make it turn blue. Hypothesis: If we add pBlu to E. coli bacteria, then it will turn blue because the pBlu will code for the color gene. Materials: 4 pipets, 7 yellow loops, 1 marker, bleach, ice, tape, hot water, 2 LB Petri Plates, 2 LB/amp/xgal plates, micropipettes and tips, and test tubes with holder. Safety: Be sure to wear goggles and wash hands and lab station thoroughly. Variables: Experimental Control: LB plate.
3. Why is it necessary to make pure subcultures of organisms grown from clinical specimens? So that the organism can be identified and tested for antibiotic sensitivities. 4. What kinds of clinical specimens may yield a mixed flora in bacterial cultures?
The purpose of these tests were to determine how the bacterium reacted to glucose with and without oxygen. My findings reported a gram stain negative along with positive tests for glucose oxidation and fermentation. Next I performed an Indole Production Test. This test determines whether the microbe produces indole from the amino acid tryptophan. The results from this test were negative.
Place tubes on ice. 4. Use a sterile plastic inoculating loop to transfer one or two large colonies of E. coli cells from the starter plate to the +BLU tube. a) Be careful not to transfer any agar from the plate along with cell mass. b) Immerse loop tip in calcium chloride solution, and vigorously tap against wall of tube to dislodge cell mass.
In the toxic mussels, the visible light absorption spectrum revealed a pattern that was characteristic of phytoplankton pigments. With further investigation, the pigments were found not to be poisonous though, and the aqueous layer consisted of the toxin. Column chromatography was used to separate the layer into organic acids and bases. Acids that were ionized quickly passed through because the resin called XAD-2 would not hold them in their polar ionized form. Out of all the acids that passed through, only one was found to be toxic.
Protects the bacteria from phagocytosis allowing the bacteria to stay in the body 6. pure culture 7. It is differential based on hemolysis of the agar. Hemolysis can be wide-narrow band beta, alpha, gamma, or none. 8. candle jar in microbiology is used for anaerobiosis in which a lit candle is placed in an air tight jar and if it went out, it would be because it used up all the available oxygen. 9. any streptococcus capable of hemolyzing erythrocytes, classified as α-hemolytic type, producing a zone of greenish discoloration much smaller than the clear zone produced by