Purpose (5 points): The purpose of this lab is to learn how to extract DNA and to analyze extracted DNA. This lab allows the conductor of the lab to analyze the steps taken to extract the DNA and realize the purpose of each step. This lab activity teaches one how cell barriers can be broken. Hypothesis: If the enzyme, alcohol, detergent, alcohol, and salt are all used accordingly to extract the DNA from the split peas, then a small amount of the DNA will separate from the solution, looking like long thin strands. DNA is insoluble in alcohol, but soluble in water, so this experiment will test this scientific principle of alcohol.
Next 3 more test tubes were filled with ~4 mL of the distilled water solution were placed in the same ice bath. Finally, 100mL of Pseudomonas syringae, nutrient bath and Escherichia coli (instead of ionized water) was added to different test tubes and whether there was a phase change or not was recorded. This was treated as the student designed test. Results: In the preliminary test it was determined that when Pseudomonas syringae was mixed with distilled water the solution formed ice crystals. But there
Next I observed the isolation streak on my Blood Agar Plate and found pinpoint, round, entire and flat colony morphology as well as an alpha hemolytic reaction pattern, indicating red blood cell ion leakage which is characteristic of S. epidermidis. The final test used Mannitol Salt Agar that selects for Staphylococcus bacteria because they are salt tolerant and differentiates between species based on agar color. The agar remained red after incubation with my unknown culture indicating that the Mannitol-D sugar was not fermented. After running this series of test I can accurately conclude that my unknown #14 culture was Staphylococcus epidermidis. At age 11, Jonathan Markway was diagnosed with congenital heart disease.
Examine your living organism and determine if it is a bacteria, achaean or a eukaryote. At each step in classification, check the requirements for each category to determine where the species belongs. You then would begin to ask yourself a series of questions about the organism one question will lead to another. If the organism cannot be identified through the questions you will than need to do a gram staining process. You will look at the bacteria through a microscope exposing the bacteria's cell wall to two types of stains: a violet and a red one.
pGLO Transformation Scientific Report BY : Tom riddle pGLO Transformation Scientific Report BY : Tom riddle Introduction The aim was to conduct an experiment that genetically transformed bacteria with Biorard pGLO plasmid. The pGLO plasmid has ability for the production of the green Fluorcent protein (GFP) (Biorard2000) which is also found in jellyfishes. The experiment conducted was to transform the e-coli bacteria with jellyfish gene that code for green fluorescent protein (GFP). During the experiment procedures were undertaking for the genetic transformation, at the end if done correctly the new developed jellyfish gene in the bacteria will glow under UV light. In this experiment there were main 4 hypothesis made according to each agar plate each with a different types of genes on them.
2. What morphological structure is responsible for bacterial motility? Flagella 3. Why is a wet preparation discarded in disinfectant solution or biohazard container? Because of the Preparation of micro- organisms which can interact with the people or environment surrounding them.
When weighing the test tube and the sample compound solution. We will need to put a beaker to make the test tube fixed on the scale. But we need to zero the scale after putting the beaker on the beaker. * Materials: 1. A large amount of ice 2.
Then place the beaker below the runoff. It only takes one filling of ice to do this experiment. A physical property that would help determine is separating the method to use for homogenous and heterogeneous mixtures is the size in the boiling points, and the color. 4. Then place the water in the 25ml beaker for the heating bath and lower the set-up so that when the 25ml round bottom flask is immersed the beaker is about 2/3 full.
First I made a water bath by filling the 100 mL beaker with cool tap water. I then placed crushed ice in the 100 mL beaker so the water level was just below the top of the beaker. I sprinkled a little salt in the ice water and mixed it well. I then filled the test tube half full with distilled water and set the test tube in the 24 well plate. I inserted the digital thermometer into the test tube and took reading every 30 seconds until the readings remained constant.
Courtney Sever Period 2 10/11/12 Experiment 3: Beer’s Law Purpose/Hypothesis: The reason we prefer to express the law with this equation is because absorption if directly proportional to the other parameters as long as the law is obeyed. In this experiment we are going to test different solutions and then test an unknown. Materials: CBL Colorimeter Test tubes (6( Test tube rack 8 Cuvettes Pipets Gatorade (unknown) Blue Stock solution Water Beakers Paper Towels Procedures: * Turn on the CBL, and open the apps to the Colorimeter setting. * Fill one cuvette with water, making sure the outside is completely dry, and set in the colorimeter to calibrate it. * Then label each test tube, 1-6.