Biology Lab Report Glowing Bacteria

2353 Words10 Pages
1-2-09 Glowing Bacteria Genes are pieces of DNA which hold the instructions for making proteins. The protein will then give an organism a particular trait. Genetic transformation means changes caused by genes, and requires the insertion of a gene into an organism, in order to change the trait. this technique is used by in many areas of biotechnology. Agriculturally, plants can be genetically transformed to be resistant to pesticides, spoilage, and frost. In bioremediation bacteria can be transformed to be able to digest oil from oil spills. For medicinal purposes, gene therapy is being used to help people with diseases caused by defective genes. The GFP protein is a green fluorescent protein that is the source for bioluminescent jellyfish. This protein causes the jellyfish to glow in the dark. With gene transformation the bacteria will acquire this trait and they wil glow underneath an ultraviolet light. Bacteria contain a large singular chromosome and one or more smaller circular pieces of DNA called plasmids. these plasmids usually contain one or more genes that are beneficial to the survival of the bacteria. in nature, the bacteria can transfer these plasmids from bacteria to bacteria, transfering those beneficial genes. this mechanism allows bacteria to adapt to new and foreign environments. the continuous resistance of bacteria to antibiotics is due to the transmission of these plasmids. Bio-Rads unique pGLO plasmid encodes the gene for GFP and a gene for resistance the antibiotic ampicillin. pGLO can also control the gene expression of this fluorecent protein. This GFP protein can be switched on when in the presence of arabinose sugar. transferred cells will appear white on the plates not containg the arabinose, and fluorescent green when arabinose is present. Data and Results: Conclusion: the control plates consisted of the -pGLO LB
Open Document