Calonna Taylor Professor Osborne BSC2010C 9 February 2014 Beyond The Cells Purpose: The purpose of this lab report is to become familiar with the use of a compound microscope and the Gram Stain. Hypothesis: If you do not know how to use a Microscope or how to Gram Stain, then your results of your lab will come out inconclusive or just wrong. Part A: Cell Structures Materials & Methods: Materials: * Colored Pencils * Paper * Access to a scanner Methods: 1. View video “Using a Microscope.” 2. Look at the slides and identify the cell structure within.
Bacteria reproduces by most commonly occurs by a kind of cell division called binary fission. Binary fission results in the formation of two bacterial cells that are genetically identical. 6. The Archaea these microbes are prokaryotes, meaning that they have no cell nucleus or any other membrane-bound organelles within their cells. 7.
They are noninfective and are intracytoplasmatic which means it takes place within the cytoplasm of a cell. They are obligated intercellular which means they cannot reproduce outside their host cell. The reticulate body divides by binary fission to make smaller RB’s and when it’s filled it begins to convert back into elementary bodies and once again becomes infectious. As I mentioned earlier Chlamydias lack the ability to synthesize ATP on its own and depends on the host cell to
Genetic Transformation of Escherichia coli with pGLO Ahmed Islam Abstract Aim: This experiment is designed to help understand the concept of genetic transformation. This is the uptake of DNA fragments from the environment by a competent bacterium. Competency must be induced in bacterium such as Escheria coli. Also, this lab helps understand the concepts of plasmids, specifically pGLO, and their genes, specifically green fluorescent gene (GFP). Expression will be regulated using promoters.
Burst the cellular membrane and the nuclei What is the name of the process used to amplify DNA? Polymarse Chain Reaction How is the amplified DNA sorted? By size What # did the DNA profile match? #3 Why was Greg’s DNA profile in CODIS? Because all forensic scientists are required to have their DNA in CODIS Toxicology Lab: Where is vitreous humor normally located?
Even though scientists need subjects to test medicine and cosmetics, animal testing should be stopped because they don’t keep exact records, the animals do not receive any care and the animals endure horrible living conditions.Most of the animals that are tested on are not recorded. Animal testing facilities are not required to keep track of mice and rats, which conveniently make up 90% of labs. According to the ASPCA, in 2002, 1.4 million animals were recorded. Animals that are recorded are larger animals such as hamsters, guinea pigs, cats, dogs, sheep and primates. Only 10% of all animals are recorded yearly.
Cloning today has not yet been perfected. Dolly the sheep was the first healthy clone; it had 276 failures before it was successfully cloned. “Dr. Rawlins thought that humans would eventually be cloned, it’s a matter of technique and time, the genie is out of the bottle.” However in the Brave New World “Standard men and women; in uniform batches “are created in “The whole of a small factory staffed with the products of a single bokanovskified egg”. Why can’t we do this today?
An Investigation into One’s Genotypic Frequency of the D1S8o Allele by way of the Polymerase Chain Reaction and Polyacrylamide Gel Electrophoresis Analysis (Alex Devlin, May 17, 2011 Kitchener Waterloo Coligate And Volcational School) Abstract: This lab was done to determine the frequency of our D1S80 allele. The methods that were taken to perform this experiment were DNA isolation, Polymerase Chain Reaction (PCR), and Gel Electrophoresis. In the DNA isolation we used Chelex and Proteinase K to strip the DNA. A thermal cycler, vortex and centrifuge were used to help remove the metal ions and keep the DNA sample mixed. During PCR the DNA was amplified using a primer and reaction mix, multiplying the amount of DNA in the sample.
* Question 1 0.5 out of 0.5 points | | | When a tRNA has an attached amino acid it is said to be chargedAnswer | | | | | Selected Answer: | True | Correct Answer: | True | | | | | * Question 2 0.5 out of 0.5 points | | | Meselson and Stahl used bacteria grown in different nitrogen isotopes to show that DNA replication is semi-conservative.Answer | | | | | Selected Answer: | True | Correct Answer: | True | | | | | * Question 3 0.5 out of 0.5 points | | | At the terminal end of a bacterial mRNA transcript a portion of the RNA loops back on itself in a formation known as aAnswer | | | | | Selected Answer: | Hairpin | Correct Answer: | Hairpin | | | | | * Question 4
The cell can generate 144 ATP molecules from just one fatty acid molecule. Because fatty acids are oxidized inside the mitochondria, acetyl-CoA can enter the citric acid cycle immediately. Acetyl-CoA initiates the TCA cycle. Pyruvic acid, two 3-carbon molecules, reacts with NAD and CoA to produce CO2, NADH, and acetyl-CoA. The acetyl group is transferred from CoA to oxaloacetic acid to create citric acid (the 2-carbon acetyl group attaches to the 4-carbon molecule of oxaloacetic acid which begets the 6-carbon molecule of citric acid).