******************************************************************************************** Answer Sheet—Module 7 Lab DNA Extraction Click on the following link and view the DNA extraction: http://learn.genetics.utah.edu/content/labs/extraction/ 1. What is the source of the cells used in this demonstration? A human. 2. Give three practical uses of DNA that is extracted: a.
Some existing knowledge is that when all of the solutions are mixed together in the test tube they will separate into three layers one consisting of the strawberry puree (lysis solution), another is the DNA precipitate, and the last is the actual DNA. (“How to Extract DNA from Strawberries”). The prediction is that the DNA will look like twisted strands of string when looked at under a microscope. Materials and Methods There were many steps taken to perform this experiment. The materials used were: * Strawberry * Plastic Bag * Cell Lysis Solution * DNA Precipitate Solution (Cold) * Test Tubes * Graduated Cups and Cylinder * Funnel * Coffee Filter * Wood Splint * Slide and Coverslip * Microscope The Plastic bags were filled with strawberries.
Restriction Digest Lab Write-Up Background A restriction enzyme is an enzyme that cuts DNA at specific recognition nucleotide sequences known as restriction sites. To cut DNA, all restriction enzymes make two incisions, once through each sugar-phosphate backbone or strand of the DNA double helix. Restriction enzymes can also be called scissors. These enzymes are found in bacteria and probably evolved to provide a defense mechanism against invading viruses. Inside a bacterium, the restriction enzymes selectively cut up foreign DNA in a process called restriction; while host DNA is protected by a modification enzyme.
DNA also contains genetic information called genes and is organized into x-shaped structures called chromosomes. Forensic Scientists can establish the identity of an individual by testing the blood, hair, saliva, skin, and semen and matching it to a DNA sample. I feel that mandatory DNA testing at birth should be passed as a new law. There are many reasons to make DNA testing mandatory at birth. The issue of paternity fraud is the most talked about.
Biological Evidence Darryl Arnold Jr. CJS / 215 Week 2 James Anderson May 1, 2015 What is Biological Evidence and what in the world is it used for? It is safe to say it is used for personnel identification. The collection of this evidence is significant to the scene. The presence of a certain fluid may be of significance in and of itself. This is how scientists and investigators get the necessary DNA to link to a suspect or victim.
Southern Blotting Laboratory Paper Abstract Southern Blotting1 allows the detection of specific molecules among the mixture separated by gel electrophoresis. Molecules are transferred from the gel to a porous membrane (nitrocellulose/nytran) by capillary action using absorbent paper to soak solution through the gel and the membrane. For DNA, specific sequences are detected in the membrane by molecular hybridization with labeled nucleic acid probes. In this laboratory, Southern blotting was used to confirm that the clones (White 1 and White 2 colony) generated in the previous experiment were from the phage λ DNA. The precise fragment of the phage λ DNA that has been inserted into the plasmid pUC18 vector is determined to be the 5.8 kb fragment.
Here one can review a discussion on the company, the medication, regulatory agencies, whistleblower laws and strict liability regarding this case. The Federal Food, Drug, and Cosmetic Act (FDCA) gives the Federal Drug Administration (FDA), a regulatory agency, the power to “regulate the testing, manufacture, distribution, and sale of drugs” (Cheeseman, 2010, p. 695). The FDA gave Pfizer the approval to sell Bextra for certain purposes and only in certain doses. The whistleblower in the Bextra case, Kopchinski, alleges that Pfizer “promoted Bextra for uses and in doses that far exceeded what the FDA had approved” (Bextra whistleblower case started investigation of Pfizer, 2009, para. 4).
It refers to differentiating bacteria beyond the species or subspecies lv. It analyzes the DNA sequences that code for the RNA portion of the ribosomes, the important protein synthesizing parts of cells. These sequences in particular are analyzed because of their consistency: Listeria monocytogenes: DUP1042B ** to continue the game after entering the ID#, you must click the Lab tab on the site and then click continue 3. Has an outbreak occurred? Yes 4.
If Neanderthals ever walk the earth again, the primordial ooze from which they will rise is an emulsion of oil, water, and DNA capture beads engineered in the laboratory of 454 Life Sciences in Branford, Connecticut. Over the past 4 years those beads have been gathering tiny fragments of DNA from samples of dissolved organic materials, including pieces of Neanderthal bone. Genetic sequences have given paleoanthropologists a new line of evidence for testing ideas about the biology of our closest extinct relative. The first studies of Neanderthal DNA focused on the genetic sequences of mitochondria, the microscopic organelles that convert food to energy within cells. In 2005, however, 454 began a collaborative project with the Max Planck Institute
Biology DNA Report 1. What is a DNA Profile? Fingerprinting technology, which is known as DNA profiling, can be used to identify individuals. Modern DNA profiling, STR analysis, only needs a few skin cells, a hair root or a tiny amount of blood or saliva. DNA profiling is useful for solving crimes but can also be used to confirm if people are related to each other.