MacConkey agar was used to help determine this. The last test that helped determine this bacteria was the Methyl Red Voges-Proskauer broth. This test came back negative. In conclusion, I believe that the unknown bacteria number two to be Enterobacter aerogenes. In comparison of the descriptive chart and the comparative analysis sheet, 18 out 18 of the tests performed were alike.
Viruses j. C. Fungi k. D. Bacteria Ans: A and D 6. A bacterial cell is found to be motile and resistant to high temperature and phagocytosis. This cell probably has: l. Endospore, flagella, capsule 7. Nitrogen is a major bioelement that can be found in: m. a. proteins n. b. Nucleic acids o. c. Polysaccharides p. D. Lipids Ans: a and b 8. All of the following are polysaccharides except: q. Glucose 9.
The two nucleotide strands that make up DNA run in opposite directions, one runs from 3’ to 5’ and the other runs from 5’ to 3’. The 5’ end is where the phosphate is positioned and 3’ is where the deoxyribose sugars are. The organic bases (base pairings) are split up into two groups; pyrimidines and purines. The pyrimidines are made of a single six-sided ring and in terms of DNA the bases that apply to this group are cytosine and thymine. Purines however are made of a six-sided ring joined to a five-sided ring.
The two heavy chains are about 400 amino acids long, and the two light chains about half that long. Each heavy chain has a hinge region where the antibody is bent, giving the monomer a T or Y shape Y 4. Activity 4: Western Blotting Technique Lab Report 1. Describe why the HIV Western blot is a more specific test than the indirect ELISA for HIV. Your answer: The HIV Western blot has a discrete protein band that represents the specific antigen the antibody is recognizing, while the ELISA uses a well that corresponds to a mixture of antigens.
In Microbiology Lab 3 I chose the unknown culture #14 and ultimately identified it as Staphylococcus epidermidis bacteria. On a microscopic level I found the organism to be gram positive with a coccobacillus shape and both tetrad and cluster cell arrangement. I performed an isolation streak with S. epidermidis on Nutrient Agar which resulted in pinpoint, round, entire, and flat macroscopic morphology. I took a loopful of the organism from the Nutrient Agar and placed it on a slide to perform the catalase test. I added a few drops of 3% Hydrogen Peroxide and it resulted in bubble formation.
Purpose In this lab we are trying to get a broader understanding of the transformation of bacteria by exposing them to pBLU plasmids. Introduction Transformation is the manipulation of a bacterial cell's DNA in order to alter the cell's genotype or phenotype by absorbing free DNA from its surroundings. This can result in a nonpathogenic bacteria becoming pathogenic by absorbing the DNA of a broken open or dead pathogenic bacteria. In our case it is taking in the pBLU plasmid. A plasmid is a spherical self-replicating DNA molecule that is not actually a part of the bacterial cell but can integrate itself into the bacterial chromosome.
To determine these percentages however, an indirect measurement was performed where RuBisCO is converted on a total protein basis assuming the chlorophyll: total protein mass ratio is 0.0421. Therefore, Losh et al. conducted experiments where RuBisCO was measured with Quantitative Western blots using an antibody which binds to a conserved region of the large subunit of RuBisCO. They concluded that RuBisCO represented < 6% of total protein in eight species of microalgae. Furthermore, they concluded that unlike in plants, RuBisCO does not account for a major fraction of cellular nitrogen in
During the PCR reaction, the forward and reverse primers anneal to the complementary sequence on the DNA and a probe that is labelled with a 5’ dye and a 3’ quencher, anneals to the target gene of the bacterium’s DNA. During PCR amplification. The probe is then cleaved and the 5’ dye is then separated from the 3’ quencher, allowing the dye to emit fluorescence. Emission of fluorescence at elongation indicate the presence of the target
Exercise 10 Gram Stain Gram staining, a differential staining technique, is one of the most important methods used by microbiologist. The technique allows us to separate bacteria into two fundamental groups; Gram positive and Gram negative. The differences between these two groups rests in the chemistry of their cell wall. Gram positive cells have a cell wall composed of a very thick layer of peptidoglycan, a uniquely bacterial molecule. While, Gram negative bacteria have a cell wall composed of a thin layer of peptidoglycan surrounded by an outer membrane.
The authors reported that the same factor also partially promotes the formation of distromatic thalli of U. pertusa and other Ulva species, highlighting the potentially important role of thallusin for the normal development of green macroalgae. Pure thallusin strongly induced the differentiation of M. oxyspermum, even at very low effective concentrations between 1 fg mL-1 and 1 ag mL-1 (Matsuo et al., 2005; Gao et al., 2006). Although thallusin can be obtained from bacterial cultivations, Nishizawa et al. (2007) undertook the total syntheses of (±)-thallusin and its analogues to allow a detailed examination of thallusin’s biological activity. Whereas the compound