The Importance of pure culture

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The Importance of Pure Culture Pure culture is a microorganisms they are everywhere from the air we breather to the food we digest also within our bodies. What is Pure Culture? “Pure culture is a population of identical cells originating from a single cell.” (4). The importance of pure culture to biological and mechanical components of manufacturing processes. It is involved in the manufacturing process in the food industry and in the pharmaceutical process. Both raw materials and final medicines and food products will contain microorganisms unless measures are emplaced for removal. In medicines the preparation of sterile products is an expensive procedure with skilled machines. There is some consideration needed to ensure the manufacture of the product is such, as the microorganism has not been contaminated. It is necessary to prevent contamination with other bacteria since: there may be competition for nutrients; the required enzyme may not be produced as readily; the end product may be contaminated and unsafe. The required enzyme that is finally produced must also be isolated from the microbial cells (5) Pure Culture techniques: • isolation • cultivation • preservation • cultivation of anaerobic microorganisms Isolation Techniques Microbiologists have developed techniques and equipment to isolate and grow pure cultures of microorganisms with no contaminations. They are used to help to identify bacteria within a system and isolate by miniaturization then substrate utilization screening methods to isolate the microbial systems by first inoculate media to separate cells. This way of isolating the system is used to easily identify the expression that express of the microbial phenotype (cell size and shape). Also individual cell multiplies to produce visible mass of cells. Once this is done an isolated colony represents reproduction

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