The Effect of Different Dilutions of Yeast Suspension on the Number of Yeast Cells Per Cm3

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Title The effect of different dilutions of yeast suspension on the number of yeast cells per cm3. Aim To estimate the effect of different dilutions of yeast suspension on the number of yeast cells per cm3. Research question Do different dilutions of yeast suspension (10-2x, 10-3x, 10-4x, 10-5x, and 10-6x) affect the total number of yeast cells in 4 primary squares of haemocytometer? Introduction “The name Saccharomyces means ‘sugar fungi’. Yeasts are saprotrophic, unicellular fungi that occur everywhere sugar solution is available – in flowers, on the surface of fruits, on leaves and stems where sap is exuded. They are also found in the soil and on animal mucous membranes”.[1] Haemocytometer is a heavy glass slide that is used to count the number of cells under a light microscope. To know the size of population of yeast cells that used in this study, it is an appropriate to use this method as it is the cheapest way to count the number of yeast cells.[2] Hypothesis As the number of multiples of dilutions of yeast suspension increase, the lower the total number of yeast cells found on the 4 primary squares of haemocytometer. This is because; the total volume of concentrated yeast suspension that has been used is only at a volume of 1 ml, while there are five series of smaller dilutions that has been carried out. Thus, it resulting in less concentrated volume of yeast suspension produced as the multiple numbers of yeast suspension increase. Variables Independent variable: Different multiples of dilution of yeast suspension which are 10-2x, 10-3x, 10-4x, 10-5x, and 10-6x. Dependent variable: Total number of yeast cells in 4 primary squares of haemocytometer Controlled variable: 1) Light intensity of microscope - The light intensity of the microscope is adjusted using diaphragm until suitable

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