Apple Juice Synthesis Lab

1043 Words5 Pages
Brendan Dennis 2/24/13 CP Biology Enzymes Used vs. Amount of Apple Juice Produced Lab Report Title: Enzymes Used vs. Amount of Apple Juice Produced Problem: How does a change in the enzymes used affect the amount of apple juice produced? Hypothesis: If different enzymes, such as pectinase and cellulase, are used to break down apple sauce, then the most apple juice will be produced when 5 drops of both pectinase and cellulase are used in one mixture because the two enzymes will be working together to break down the apple juice. Procedure: 1. Gather all necessary materials. 2. Proceed to assigned lab station. 3. Once at assigned lab station, put on safety goggles to protect eyes, apron to protect clothing, and tie back all lose items. 4. Fold coffee filters into quarters, then place into four glass funnels. 5. Put funnels with coffee filters inside wooden block on ring stand 20 cm off table. 6. Place one ounce of applesauce into each filter using a spoon. 7. Collect 10 drops of pectinase, cellulase, and distilled water from teacher. 8. Proceed to carefully place 10 drops of pectinase into first funnel. 9. Pour juice produced after set time into graduated cylinder then record amount. 10. Carefully place 10 drops of cellulase…show more content…
Analysis: My conclusion, that the change in enzymes will affect the amount of apple juice produced because each enzyme will work differently with the apple sauce, can be proved with the data from this experiment. It can be proved because when the enzyme was changed different amounts of apple juice were produced. An example of this could be the pectinase and cellulase solutions. When the cellulase was added to the apple sauce it produced only 1 mL of apple juice, but when the pectinase was added to the apple sauce it produced 4.5 mL of apple juice. That is a change of 3.5 mL from one enzyme to another. This data obviously proves that a change in the enzyme produced a different amount of apple

More about Apple Juice Synthesis Lab

Open Document