Strawberry Dna Lab Write Up

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Strawberry DNA Purpose: To extract DNA from the fruit of a strawberry plant. Background: In this lab we extracted DNA from a strawberry. DNA, or, deoxyribonucleic acid, is the hereditary material in humans and almost all other organisms. DNA is located in the nucleus of the cell, and is made up of four chemical bases: adenine (A), guanine (G), cytosine (C), and thymine (T). Materials: * Heavy duty zip-lock baggie * 1 strawberry (fresh or frozen and thawed) * Cheesecloth * Funnel * 100 ml beaker * Test Tube * Wooden coffee stirrer * DNA extraction buffer * Ice-cold 95% isopropyl alcohol Hypothesis: DNA can be extracted if you use certain buffers. Procedure: 1. Place one strawberry in a zip lock baggie and carefully press out all of the air and seal the bag.…show more content…
Smash the strawberry with your fist for 2 minutes. 3. And 10 ml extraction buffer to the bag and carefully press out all of the air and seal the bag. 4. Mush again for one minute. 5. Filter through cheesecloth in a funnel into beaker. Support the test tube rack. 6. Discard the extra mashed strawberry. 7. Pour filtrate into test tube so that it is 1/8 full. 8. Slowly pout the ice-cold alcohol into the tube until the tube is half full and forms a layer over the top of the strawberry extract. 9. At the interface, you will see DNA precipitate out of the solution and float to the top. You may spool the DNA on your glass rod or pipette tip. 10. Spool the DNA by dipping a pipette tip or glass right where the extract layer and alcohol are in contact with each other. With your tube at eye level, twirl the rod and watch as DNA strands collect. Data: | Amount Added or Obtained | Initial Color | Purpose | Buffer | 10 ml | blue | Break through the cell membrane | Strawberry | 1 | red | Subject tested
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