Purpose The purpose of this experiment is to see the effect that paper chromatography has on ink. Materials • Four different markers (including one black permanent marker) • Rubbing alcohol or isopropyl alcohol • Coffee filters (2) • Tall glasses or plastic cups (2) • Pencil • Ruler • Tape • Table salt • Water • Measuring cups/spoons • Clean pitcher or 2-liter bottle Procedure 1. Coffee filters usually are round, but it's easier to compare your results if the paper is rectangle. So, your first task is to cut the coffee filter into a rectangle measuring three cm by nine cm. You will need two of these strips to complete the lab.
I then poured the mixture on to a solid white piece of paper. I then used a magnet wrapped in clear plastic bag to separate the iron from the mixture. I weighed the iron and calculated and recorded the mass in table 1. I put the remaining mixture in a beaker with 50 mL of distilled water and heated to a boil and removed, then poured the liquid from the beaker into a paper cup. I then added 15 mL of distilled water and heated to a boil and removed, then poured the liquid from the beaker into the same paper cup that held the previous liquid.
Added 2-3mL of liquid household bleach solution and stirred; solution turned orange. 5. Dipped cotton swab into the salicylic acid hydrolysis solution and wrote a message on a blank piece of flat, white paper and allowed it to dry. 6. Laid the dried message paper on several layers of paper towels for the visualization step to keep the developer solution from soaking on the work surface and surrounding materials.
magnets zipper locking plastic bags beakers Petri dishes micro spatulas tongs 13- x 100-mm test tubes; rubber or cork stoppers to fit rings, ring stands, gauzes, burners, ignitors ice paper towels filter paper funnels Using this equipment, devise a procedure for the separation of the four substances. Design experiments, and write procedures for the designs. Ask the instructor to approve each experimental design. Questions Explain why the sodium chloride is contaminated with benzoic acid. Suppose sawdust from wood less dense that water were incorporated into the mixture.
Practice spotting on a piece of filter paper. Use toothpicks to apply a drop of each cation solution in the appropriate columns, Place the paper in the 600 mL beaker, then immediately re-cover it, Without agitating the solvent, allow the solvent front to rise within 1 cm of the top edge before removing the plastic cover. Perform 3 spot tests, and record the results on Data Sheet 1. Once the solvent front is within 1 cm of the top edge of the paper, remove the paper and mark the final position of the solvent front with pencil. Re-cover the beaker, and completely dry the paper.
Hypothesis: the colors will separate into yellow and green. III. Materials: isopropyl alcohol, green leaf, coffee filter, coin, beaker, ruler, scissors, pencil, tape, colored pencils or crayons. Procedure -Obtain a strip of chromatography or cut 2 ½ cm strips from a coffee filter. -Use a ruler to measure and draw a light pencil line 2 cm above the bottom of the paper strip -Wrap a leaf around a coin with the waxy side of the leaf facing outward.
Rf = Ds/Df Ds = Distance traveled by the compound Df = Distance traveled by the solvent Materials: coffee filter, tape, isopropyl alcohol (rubbing alcohol), water, 3 different color magic markers (not permanent); 3 identical tall, narrow drinking glasses; metric ruler, 3 pencils Pre-lab Questions: 1. List one extensive and one intensive property of marker ink. Intensive: color Extensive: volume 2. Define mixture. Two or more solutes in a solvent forms a mixture 3.
Add two drops of food coloring to the beaker of distilled water and mix thoroughly. Measure out a dropperful of bleach into a plastic pipet and set it aside until Step 8. 6. Calibrate the colorimeter a. Prepare a blank by filling an empty cuvette ¾ full with distilled water.
Stirred the mixture to make sure all soluble material dissolves. 10. Poured the liquid while it is hot into a small paper cup. 11. Poured another 10 to 15mL of distilled water into the beaker containing the sand, brining the mixture to a boil and decanted again into the same cup used previously.