Experiment 11 Lab Assignment Answer Sheet: Chromatography of Food Dyes Name: Pre-Lab: Define the Rf value of a compound The "rf" value is the distance the compound moves relative to the distance the solvent moves. Data and Observations: Record the distance travelled by the sample and the solvent in the table below and calculate the Rf values. Table 1. Color Distance(m Blue1 Blue2 Red3 Red40 Yellow5 Yellow6 33 3 10 22 42 30 .55 .05 .17 .37 .70 .50 m) Rf Solvent 60 Table 2. Kool-Aid®: Grape Kool-Aid®: Strawberry Distance (mm) Red: 5 10 Distance (mm) Blue:17 n/a Rf .08 and .28 .17 Substance Solvent 60 Table 3.
Why? Experiment 2: Starch Test Fill in the table below with the results from the starch test experiment. Results Starch Test Solution Initial Color Color with Iodine Solution starch solution water Iodine solution is added to a chocolate chip cookie solution. The color changes to purple. Based on this result, what biological molecules are present in the chocolate chip cookie solution?
Experiment 11 Lab Assignment Answer Sheet: Chromatography of Food Dyes Name: Pre-Lab: Define the Rf value of a compound: It is the distance traveled by the compound divided by the distance traveled by the solvent front. Data and Observations: Record the distance travelled by the sample and the solvent in the table below and calculate the Rf values. Table 1. Color|Blue1|Blue2|Red3|Red40|Yellow5|Yellow6|Solvent| Distance(mm)|50mm|0mm|10mm|21mm|28mm|23mm|50mm| Rf|1|0|0.2|0.42|0.56|0.46|1| Table 2. Substance|Kool-Aid®: Grape|Kool-Aid®: Strawberry|Solvent| Distance (mm)| 19mm(red)|28mm(red)|48mm| Distance (mm)| 47mm(blue)||48mm| Rf|0.39 & 0.98|0.58|1| Table 3.
As we know the equation C1V1=C2V2, we can get a set of concentration (g/L) of different ratio of Red #40. Introduction The goal which the experiment designed to achieve is perform a Beer’s law analysis to determine a solution’s concentration, and determine a percent composition. To build the calibration curves for each food dye, we need to measure the absorption of different solutions of known concentration. One rather quick way to make a solution of known concentration is by exact dilution from a more concentrated solution of known concentration. We rinsed a pipette with some of the sample, filled curettes between 2/3 and 3/4 full with the samples, put a curette in the cell holder and make absorbance measurements.
Running head: M&M PROJECT REPORT Abstract This paper examines the proportion of M&M candies through the random sampling of 156 bags. After reading this paper one should have a better understanding of the process that accompanies packaging M&M candies. The bags used in the experiments were taken from different stores to help ensure a true sample of all the 1.69 oz of plain M&M candies. Introduction: Purpose of Report The purpose of this report is to examine the five project parts of an M&M analysis and examine a method for quality control. The parts of this project included using random sampling to gather data on the number of colored M&M candies, the sample proportion and sample mean, constructing a 95% confidence interval and testing claims of M&M candies.
Caloric Content of Food obseRvations & Calculated Values (Calculated values have the yellow background.) Data Table 1: Food Item - Observations | Food Item Description: | Marshmallow | Peanut / Walnut | Chip | Mass of food & holder – initial | 6.5g/26.5g | 1.1g/26.2g | 1.2g/26.2g | Mass of food & holder – final | 32g | 26.8g | 26.8g | Mass of food burnt | 5.5g | 0.6g | 0.6g | Mass of beaker | 65.5g | 65.5g | 65.5g | Mass of beaker & water | 109.3g | 109.3g | 109.3g | Mass of water | 44g | 44g | 44g | Water temp. – initial | 20oC | 22oC | 18oC | Water temp. – final | 29oC | 38oC | 25oC | ΔT (oC change) | 9oC | 16oC | 7oC | Heat Energy gained by water | 1656.86 J | 2945.54 J | 1288.67 J | Heat Energy per gram | 301.25 J/g | 4909.23 J/g | 2147.78 J/g | QUestions Which of the foods tested contains the most energy per gram? Peanut How do your experimental results compare to typical experimental values (listed in the procedure)?
Experiment Date: 11/22/2014 Date Submitted: 11/25/2014 TITLE: Caloric Content of Food PURPOSE: To measure the energy content of various food items, as well as become familiar with energy units like calories and joules. PROCEDURE: First, weigh your empty beaker, fork, beaker with half water, mass of marshmallow walnut popcorn, and the test tube holder. Then record the temp of the water in the breaker. The next thing you do is light the marshmallow on the fork and hold under the beaker making sure to stir the water occasionally. Once the marshmallow is completely burnt, record the temp of the water and weigh the fork with the marshmallow.
As a more polar solvent is use to push the different rings of pigment, these are collected in their own test tubes to then be run in a TLC which will determine the polarity using the Rf values and then comparing them to the table in the organic lab manual ones. This is important because we want to know what some things are composed of and by using the polarity in different components makes it easier to determine them. The gain from this experiment is determining the different pigment of spinach and by doing so comparing the polarity of each of them. Then determine why that could be by looking at the structures of the pigments separated. Discussion: The first thing done in
2. Pour milk into the Petri dish. 3. Add the food coloring into the milk by placing one drop of each color in a triangle formation. 4.
Experiment and Observations I collected all my materials together; distilled water, toothpicks, clear tape, Gatorade blueberry-pomegranate, glass beaker 100 mL, colorimeter assembly – PVC pipe on foam block with cap, 9-volt battery, jumper cables – NOT IN LABPAQ (2), FDC blue dye #1 – 2 mL in pipet, 2- 12 well test strips, pipet, fine tip, multimeter – digital, 5 test tubes, wax pencil, test tube rack. First I obtained pure M and then prepared a Beer’s Law plot with the data. I then obtained the percent transmission of the various samples. Next I obtained the percent error using the theoretical and actual values. There was a 36% error and Company Q was not in compliance with the law according to their concentration values.