Rate of Absorbance in Catechol

1054 Words5 Pages
Observing the Rate of Production in an Enzyme (Catecholase) Catalyzed Reaction and Measuring the Absorbance of Light at 70 degrees Celsius Alicia Lopez Abstract Write at the end Introduction The purpose of my group’s experiment is to measure the rate of production of the enzyme found in potatoes. The enzyme is called Catecholase. When I combined potato extract with catechol, the chemical reaction that occurs is called benzoquinone. Benzoquinone is the reddish brown stuff found in potatoes. My group’s hypothesis is if that if we raise the temperature of the enzyme and the potato extract then the reaction rates will be considerably higher and more light will be absorbed when measured. The heating variable will denature the protein. In the experiment, I raised the temperature of the Catechol and the enzyme from room temperature to 70 degrees Celsius separately. I then combined the heated extract with the catechol and measured the amount of light that was absorbed by the product, benzoquinone. I timed it in 20 second increments and noted the result in a spreadsheet. Materials and Methods Our group consist of myself, Lydia Huber, Erin and Ashley. The experiment was conducted in Cell Biology for Health Lab class on July 22, 2009. For comparison purposes and experience, prior to the experiment we conducted a similar experiment without the heating variable. This also allows us to become familiar with a machine familiar called the spectrophotometer. It measures the transparency of an object or solution by shining a beam of light through it. The product that is made during the reaction absorbs light. We set the mode to Transmittance and the wavelength to 540nm as instructed by the instruction sheet provided by our instructor. The first thing we did is to make sure we had all the necessary tools and chemicals

More about Rate of Absorbance in Catechol

Open Document