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DATE: 9th AUGUST 2011 TITLE: INVESTIGATION OSMOSIS IN QUAILS’ (Coturnix japonica) EGG Research Question How does different concentration of sodium chloride solution which is 0%, 5%, 10%, 15% and 20%, affect the mass of de-shelled quail’s eggs after they are immersed in sodium chloride solution for 1 day(24 hours)? Variables Table 1: Independent and Dependent Variables Variables | Units | Range | Independent variable | Concentration of sodium chloride solution | mililitre(ml) | Concentration of 0%, 5%, 10%, 15% and 20%. | Dependent variable | Change in the mass of quail’s egg | gram(g) | Difference between initial and final mass of quail’s egg | Table 2: Controlled Variables and Its Possible Effects On The Experiment. Controlled variables | Units | Possible effects | Time taken of the quail’s eggs are being immersed | Hours | The long time taken of the quail’s egg are being immersed in the sodium chloride solution which is 24 hours is kept constant.This causes the change of mass of the quail’s egg resultant from the osmosis process. | Volume of sodium chloride solution used | Millilitre(ml) | The volume of sodium chloride solution is kept constant so that the water molecules in each solution are the same. | Type of eggs | Quails’ eggs | Same type of eggs is used to have the same thickness of the egg membrane which will affect on the rate of osmosis. | Hypothesis Osmosis is the tendency of water to flow from a hypotonic solution (low concentration of dissolved substances) to hypertonic solution (higher concentration of dissolved substances) across a semi-permeable membrane. ( http://www.biology-online.org/dictionary/Osmosis) When the concentration of sodium chloride solution, NaCl is 20%, the concentration of water molecules in the solution is lower than in the de-shelled quail’s egg. Thus, water molecules will diffuse from the egg out into
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