Experiment 5: Titration for Acetic Acid in Vinegar Name: Lab Partners: None Date of Experiment: March 25th, 2013 Location: My House Course Number: Chem 112 Abstract: In this experiment the objectives were develop familiarity with the concepts and techniques of titration and to determine the concentration of an acetic acid solution in vinegar. Experiment and Observation: Data Table 1: Quantity of NaOH needed to Neutralize 5 mL of Acetic AcidBrand of Vinegar Used: Heinz White Distilled VinegarLabel Notes: ______________________ | | Initial NaOH reading(Interpolate to 0.1 mL) | Final NaOH reading(Interpolate to 0.1 mL) | Volume of NaOH used | Trial 1 | 9.5 | 1.3 | 8.2 | Trial 2 | 9.7 | 1.4 | 8.3 | Trial 3 | 9.6 | 1.4 | 8.2 | Average volume of NaOH used: | 8.2 | Calculations and Errors: Errors: Calculations: A. Calculate the average number of mL of NaOH used for the 3 trials and record. B. Calculate the Normality of the vinegar using the previously given equation.
Follow all procedures in the weekly iLab page, as well as, Toolwire’s lab procedures and tips. 4. While performing your labs, capture and paste all screenshots identified in this Lab Report Template. 5. After each lab, summarize what you learned in a short paragraph – minimum three college-level and well-written sentences.
Next I observed the isolation streak on my Blood Agar Plate and found pinpoint, round, entire and flat colony morphology as well as an alpha hemolytic reaction pattern, indicating red blood cell ion leakage which is characteristic of S. epidermidis. The final test used Mannitol Salt Agar that selects for Staphylococcus bacteria because they are salt tolerant and differentiates between species based on agar color. The agar remained red after incubation with my unknown culture indicating that the Mannitol-D sugar was not fermented. After running this series of test I can accurately conclude that my unknown #14 culture was Staphylococcus epidermidis. At age 11, Jonathan Markway was diagnosed with congenital heart disease.
All results for this unknown can be found in the tables under results section of the report. The following tests were performed on unknown #15: * Gram stain | * Hydrolysis of starch | * Nitrate reduction | * Selective/differential media | * MR-VP | * Catalase/oxidase | * Enrichment media | * Citrate utilization | * DNase | * Isolation media | * Hydrolysis of Gelatin | * Esculin hydrolysis | * Gas-pak jar | * | * | * Candle Jar | * Indole production | * Triple sugar iron | * Thioglycollate tube | * Hydrogen sulfide production | * Litmus milk | * Fermentation of sugars | * Urea Hydrolysis | * Sulfur, Indole,motility | Unknown number 15 was assigned by the instructor. A differential staining
Diffusion and Osmosis Lab Part 1A: Diffusion Evidence: Table 1: The Color of Different Solutions Before and After Diffusion for 30 Minutes Content | Initial Solution Color | Final Solution Color | Bag15% glucose1%starch | Cloudy white | Very dark purple | BeakerWater + IKI | Golden yellow | Golden yellow | The above table represents the final and initial colors of the solution that was inside of the dialysis tubing and the solution that was in the beaker. A 15% glucose and 1% starch solution was placed inside of the dialysis tubing. The initial color of the solution in the bag was a cloudy white but after 30 minutes of sitting in the beaker of water and IKI the color turned a very dark purple almost black. Inside of the beaker was a water and IKI solution. After sitting for 30minutes this solution stayed the same color.
The procedure: Cabasco-Cebrian, T.; Loftus, C.; Schulz, J.; Villarba, M.; Wick, D. “Lab Manual for CHEM 162” Winter 2011, Department of Chemistry, Seattle Central Community College, pp. x-y. Data: | Test tube A | Test tube B | Run No. | 0.2% Starch Drops | 0.012 M Na2S2O3 | 0.20 M KI | 0.20 M KNO3 | 0.20 M (NH4)2S2O8 | 0.20 M (NH4)2 SO4 | 1 | 5 drops | 2.00 mL | 8.00 mL | 2.00 mL | 4.00 mL | 4.00 mL | 2 | 5 drops | 2.00 mL | 4.00 mL | 6.00 mL | 4.00 mL | 4.00 mL | 3 | 5 drops | 2.00 mL | 2.00 mL | 8.00 mL | 4.00 mL | 4.00 mL | 4 | 5 drops | 2.00 mL | 4.00 mL | 6.00 mL | 8.00 mL | 0.00 mL | 5 | 5 drops | 2.00 mL | 4.00 mL | 6.00 mL | 2.00 mL | 6.00 mL | A. Effect of Concentration Test tube | Run No.
Make sure you graph along the rigid side because fingerprints on the clear sides can mess up the readings. You then pipet the amounts of material listed in Table 1 of your lab manual, then shake the mixes thoroughly. Turn on you spectrophotometer and set the wavelength dial 540 nm. Allow the instrument to warm up for ten minutes. Then prepare the unknown sample by pipetting 1 ml of the unknown solution into the cuvette and mix with 1 ml of dilute ferric nitrate.
READING ASSIGNMENT: ● This handout for procedure . ● Supplementary information in Janice Gorzynski Smith (2 nd ed), Chapter 22.11 PRE - LAB ASSIGNMENT : ● Complete all portions of pre - lab notebook work according to the guidelines. ● Rewrite the procedure in your lab notebook using your own words. ● Sapling assignment. CHEM 51LC Page 2 Rev 04 /30 /1 3 CAUTION Sodium hydroxide is corrosive and causes burns.
Christian Oetken 2/20/15 College Chemistry Serparation of a Mixture of Solids Inductor: Today in this lab is to understand the steps involving in separation of multiple solids and how to processing the separation of iron filings, sand, table salt and benzoic acid. Results: Data Table 1: Experiment Data | | Grams | Percent of mixture | Iron filings | 1.5 | 25% | Sand | 1.4 | 23.33% | Table salt | 1.5 | 25% | Benzoic acid | 1.6 | 26.66% | Total | 6.0 | 100% | Observe how easy to separate sand and the iron fillings but hard to separate benzoic acid and salt. Benzoic only separate in cold water and salt separated in hot water so process for those two substances was longer Discussion: Lab was very successful on understanding separation of a mixture of solid. Each solid some equal one was little higher but not much and one lower but not by much. Question: A.
Observations Data Table 1: Experiment Data | | Grams (g) | Percent of Mixture (%) | Initial Mixture | 6.5 | 100 | Iron Filings | 1.8 | 27.69 | Sand | 1.7 | 26.15 | Table Salt | 1.9 | 29.23 | Benzoic Acid | 0.8 | 12.31 | Combined Total | 6.2 | 95.38 | Questions A. How did your proposed procedures or flow charts at the beginning of this experiment compare to the actual procedures of this lab exercise? The flow chart I created listed each substance and the weight, it looked similar to the one on the disc but did not have a column for percentage B. Discuss potential advantages or disadvantages of your proposed procedure compared to the one actually used. The disadvantage of my flow chart would be that the weight would have been measured but the percentage would not give the accurate scientific measurements.