Pseudomonas Aeruginosa The purpose of this lab was to identify the unknown bacteria culture given by our respected instructor using various differential tests. The identification of this unknown culture was accomplished by separating and differentiating between possible bacteria based on specific biochemical characteristics. Each test was used in a way to help recognize those specifics and bring us one step closer to identify the unknown cultures. The differential tests used to identify the unknown cultures were oxidase, lactose and glucose fermentation, citrate, indole, urease, motility, H2S, EMB and MAC. The tests performed on the unknown bacteria culture were all used to determine and identify the bacteria.
Kyla Emerine Date of Experiment: April 15, 2015 Report Submitted: August 17, 2015 Title: Colligative Properties and Osmotic Pressure Purpose: To observing changes of colligative properties in freezing and boring points, by adding salt to a solution. Procedure: In this experiment I performed various procedures such as adding different amounts of salt to tap water that was freezing or boiling and observed how it changed the temperatures. Data Tables: Data Table 1. Dialysis Tubing Results Time: |Mass of Dialysis Tubing and Contents | 0 minutes |6.5g| 30 minutes |6.8| 60 minutes |7.2| Questions A. In your experiment, is the light corn syrup in the dialysis tubing hypertonic or hypotonic to the water?
Title: 1pt Stoichiometry Of A Precipitation Reaction 2. Procedure: 2pt (Write the procedure in your own words after you have read the lab manual) Put on goggles the weigh 1.0 of CaCl*2H2O and put it in 100ml beaker. Add 25 mil of water and stir. I will then use stoichmetry to figure out how much Na2CO3 I will need for a full reaction. I will weigh this and put it in a separate cup.
Name: Jeffrey S. Webster Date: 8th October, 2007 Course: BC21D ID No. : - Lab Partners: Paul Bair, Jermaine Levy, Rochelle Allen Aim To extract and purify the enzyme invertase, thereby allowing for subsequent study of the effects of the parameters of [enzyme], [substrate], pH, temperature, and inhibitors on invertase activity. Method As outlined in the BC21D lab manual. Effect of Temperature on reaction velocity Place 1.0 ml of sucrose and 0.8 ml of pH 4.7 acetate buffer into a test tube and incubate for 6 minutes in a water bath at 37 oC. Add 0.2 ml of yeast invertase and incubate at the same temperature for 6 mins.
Add a two-gram sample of each of the above compounds to each test tube. 3. Stir the samples. 4. In the chart provided record if each compound is solubility in ethanol.
Wash and add Gram’s iodine (mordant) - 1 min. 4. Drain off iodine, add alcohol (decolorizer) -15 seconds 5. Wash and add Basic fuchsin or Safranin (secondary stain)- 1 min. 6.
Enzymes Amylase: | Tracing the Breakdown of Starch | Rashaud Pickering, Ashley Bagnis, Stephanie Alvarado, Elbany Angulo 3614098 Section U-14 | Signature___________________ Abstract An experiment was conducted to find out what the optimal temperature for amylase to break down starch was. An Iodine test was used to find evidence of starch formation. Three drops of iodine were dropped into two sets of spot plates. There were four test tubes that contained human amylase and four test tubes that contained fungal amylase. These specimens were all tested before being placed into their respective ice or water baths.
DC (NF/DJ) 27049/4 © UCLES 2011 [Turn over www.XtremePapers.net 2 Answer all the questions in the spaces provided. For Examiner’s Use 1 Ethanoic acid can be reacted with alcohols to form esters, an equilibrium mixture being formed. CH3CO2H + ROH CH3CO2R + H2O The reaction is usually carried out in the presence of an acid catalyst. (a) Write an expression for the equilibrium constant, Kc, for this reaction, clearly stating the units. Kc = units ................................................. [2] In an experiment to determine Kc a student placed together in a conical flask 0.10 mol of ethanoic acid, 0.10 mol of an alcohol ROH, and 0.005 mol of hydrogen chloride catalyst.
We then shook the jar until it was well mixed and waited about thirty minutes for the mixture to settle. Once it settled we measured the thickness of each layer of the soil. In the water holding experiment we simply filled a beaker with 100 ml. with garden soil then poured water into the beaker to saturate the soil enough so that there was no air trapped in the dirt. We measured the amount of water we used and calculated the pore space as well as the percentage of pore space.
Herbal Medicine and Its Effects on Pathogenic Bacteria Shawna Henry Roberts Wesleyan College CMP 102-01 Abstract Bacterial infections have increased in recent decades due to the resistance to treatments. Alternative treatment is herbal medicine which has been used to treat a variety of diseases and infections. Therefore, herbal medicine should be used to treat pathogenic bacteria. Sources from journal articles have proven the antimicrobial effects of plant extracts. Herbal Medicine and Its Effects on Pathogenic Bacteria MRSA, Typhoid fever, and Gonorrhea are bacterial diseases that have recently become resistant to their antibiotic treatment.