By looking at the spread sheet it conveyed that all 3 bacterials were negative for the oxidase test. So, I assumed that if the oxidase test performed came back negative as well, this would verify that I chose wisely. The oxidase test would also detect if the bacteria produced cytochrome oxidase, it is also used to segregate between aerobic gram-negative organisms. The last experiment set up on this day was the fluid thioglycollate medium (FTM) so that I could verify if my unknown bacteria was a aerobic or anaerobic
Unknown Lab Report: Enterobacter cloacae Jessica Sainvil Professor Cupido Bio 253L1 Thu 9:30-12:30 Due Date: 12/6/12 Unknown#103 Introduction The bacterium Enterobacter cloacae isolated from the given #103 was a Gram-negative Rod. The purpose of this lab was to isolate and identify the genus and species of an unknown bacterium. It is important to identify an unknown microorganism because knowing how the bacteria work and how it is structured means knowing how it can affect humans. Unknown bacteria may also be used clinically many pharmaceutical drugs are based on products made by organisms (Katzung, B.G.2004). In order to identify the unknown organism a series of tests were performed.
NH4+ Heat 5mL of the test solution in a beaker. Test the gas evolved with damp red litmus paper. The damp red litmus turns blue Ammonia gas. - 4. Ag+ Add HCl(aq) to original solution.
These being, E.Coli and S.Albus. Some of the developed antibiotics work on all forms of bacterial life, this means destroying of all bacteria it gets in contact with. Some act on a range of bacterial life, giving us the answers to which affect and do not affect the bacteria. Lastly some do not have any affect on the bacteria at all. In order to find out which antibiotics work on the bacteria, doing an agar plate test is the best way to get answers.
02 April 15, 2014 #7 Enterobacteriaceae, Escherichia coli Staphylococcaceae, Staphylococcus epidermidis ABSTRACT The objective of the Unknown Report is to identify two unknown bacterium based on a series of tests learned in the microbiology laboratory. The tests performed on organism A were Fluid Thioglycollate, Gram Stain, MR/VP, SIM, Simon Citrate, and LB plate. The results for organism A were Facultative Anaerobe for aerotolerance, Gram negative, MR positive and VP negative, the SIM test results were positive for Indole productivity and growth radiating but negative for sulfur production, Simon Citrate negative, and there was no red growth on the LB plate. These tests lead to the discovery of the bacterium Escherichia coli which
After that, dissolve the sample in 2 mL of deionized water and shake the test tube for 1 to 1 ½ minutes to dissolve the solid. Place another dry test tube in a 50mL beaker and weigh it. Find a bottle of barium iodide and record the name and molar mass. Then, weight out either anhydrous barium iodide or barium iodide dehydrate into this test tube and dissolve is it in 2 mL of deionized water. Pour the contents of one of the test tubes into the other and a reaction should occur and you should see a white precipitate of barium sulfate form.
The purpose of these tests were to determine how the bacterium reacted to glucose with and without oxygen. My findings reported a gram stain negative along with positive tests for glucose oxidation and fermentation. Next I performed an Indole Production Test. This test determines whether the microbe produces indole from the amino acid tryptophan. The results from this test were negative.
Then after agitating the seaweed to remove monosodium glutamate and pouring off the water, 40 mL of distilled water was added to the seaweed and heated. During this procedure, the iodine extract was filtered and several tests, both standard procedure and home- Objective: The purpose of this experiment is to understand how a single substance can be isolated from a mixture, like seaweed and water. Also, learn how to utilize filtration and extraction to make a tincture for testing for Iodine, Iodide Ion, and Triiodide Ion. Procedure: 1. 6 grams of dried seaweed, cut into ½ inches should be places into a 150 mL beakers that will later be filled with ¾ distilled or deionized water.
If the urea is broken down by H. pylori in the biopsy, there is a change in color around the biopsy on the slide. This means that there is an infection with H. pylori in the stomach. Biopsies also may be cultured in the bacteriology laboratory for the presence of H. pylori; however, this is done infrequently since other simpler tests are available. A recently-developed test for H. pylori is a test in which the presence of the bacterium can be diagnosed with a sample of stool. The test uses an antibody to H. pylori to determine if H. pylori is present in the stool.
General Biology and Lab I - BIO195 Problem Solving Using the Scientific Method Name: Eric Asirifi Partner: George and Kalid DATA CHART Cavity No. 1 2 A B C D X Y Z Bottle I II Solution 1 Solution 2 Solution 3 Solution 4 Solution 5 Solution 6 Solution 7 Formula unknown unknown Original Color of Brom thymol blue Blue Blue Blue Blue Blue Blue Blue Blue Blue Yellow Blue Yellow Predicted Color Blue Yellow Observations Yellow Yellow Blue Blue Yellow Blue Green Print out and hand write formulas Observation What did you observe when you added brom thymol blue to solutions 1-7? When indicator interacts with acid it turns yellow and when indicator interacts with base it turns blue. Question What is the obvious question that