Experiment Colligative Properties & Osmotic Pressure Karen Curry 1/19/2014 1:00pm Abstract The purpose of this experiment is to understand and compare the difference of the freezing points between pure solvents in a solution and a non-volatile solute. Secondly, osmosis is demonstrated in a permeable membrane in this case a dialysis tubing and a less permeable membrane with a much harder shell like the egg. Experiment and Observation Starting with Part I of this experiment I gathered together all my items I needed. Small rubber band, salt, tap water, distilled water, 1/8 teaspoon measuring spoon, crushed ice, beaker 100 mL plastic, stopwatch-digital, test tube 13 x 100 mm, digital thermometer, well plate-24. First I made a water bath by filling the 100 mL beaker with cool tap water.
Read the absorbance at 20 second intervals from the start of the mixing. Then record your measurements on the table. After two minutes remove the tube from the spectrometer and visually note the color change. 6) Now mix the contents of tubes 4 and 5, transfer to a cuvette, and repeat your measurements for two minutes at 20 second
Results and Discussion For the first part of the experiment (Part A), five different 100 mL volumetric flasks were each filled with 1,2,3,4 and 5 mL of iron (II) solution. Then 5 mL of YY ligand, were poured to each of the five flasks. Each flask had 5 mL of 2M sodium acetate and 4 mL of 3M NH2OH. Then the whole solution was diluted up to the 100 mL fill mark with distilled water. This was the solution that was used in order to obtain the absorption spectrum for each of the different iron (II) ligand examples different flasks.
1) ABSTRACT The purpose of this experiment was to carry out the effects of enzyme concentration, temperature, pH level, and an inhibitor on a type of enzyme called Perixodase. The purpose of the experiment was also to understand the relationship between the change in absorbance with time and the enzyme reaction rate. To perform the experiment, the class was divided into groups. Each group took on one of the factors affecting the enzyme. Before beginning the actual experiment, we used the mixing tables that were given to us to set up the test tubes with the appropriate substances.
In this experiment, a chromatography column was pre-created in a plastic tube containing glass beads with microscopic holes and an excess of buffer solution. After draining the initial buffer solution to the top of the column bed, 25 microliters of a hemoglobin (Hb)/vitamin B12 mixture sample were pipetted into the top opening of the column using a P200 pipette instrument. After a few minutes, the mixture began to separate within the column tube. 25 microliters of the buffer solution were then added to the column, using the P200 pipette instrument, to continue the chromatography process and prevent the column from drying. Drops from the column were collected from this point on, using 10 collection tubes labeled 1-10.
Using a graph of ln(K) vs. 1/T, Ea was found to be .00426 KJ/mol and A was found to be 2.4033 s-1. E X P E R I M E N T A L S E C T I O N Many experiments were conducted to gather data, from varying temperatures and concentrations of solutions, to find the unknown variables α, β, A, and Ea. All glassware was initially cleaned with distilled water to avoid possible confounding ions that could tamper with the reaction. In the first section of the experiment, six different trials (with the concentration of all solutions kept fixed) were conducted by varying the temperature of the solutions. 10 mL of each solution [0.16M KI, 0.0055M (NH4)S2O8, 0.12M Na2S2O3, and water] were added to an Erlenmeyer flask along with about 0.2 g of starch and a drop of EDTA (to prevent coagulation) and mixed with a stir bar.
pyridinium hydrobromide perbromide type of stationary phase column length column temperature rate flow of the carrier gas List the 4 general factors that affect the separation obtained on a gas chromatograph What specific technique is used to collect/isolate your purified unknown compound at the end of the recrystallization experiment? suction filtration 14 of 22 4/16/12 9:15 PM StudyBlue Flashcard Printing of Lab Final 2211L UGA
Out of all the acids that passed through, only one was found to be toxic. By using high performance liquid chromatography, a polar and nonpolar stationary phase that contained the toxin was mixed, and this isolated the toxin by itself. To ensure that that was the isolated toxin, they further broke it down to find its charge, size, and molecular shape. Mass spectrometry was then used to find the weight and formula of the toxin. By finding the formula, it had similar characteristics to an amino acid known as domoic acid.
6/28/2012 The 1st lab procedure performed was the gram stain. Previously, before starting the gram stain I prepared a bacterial smear on a glass slide and used the heat fixing method which was done to help the bacteria cells adhere to the glass, kill them, and also helps make the cells easier to stain by coagulating the cells proteins. The gram stain test helped me identify rather my bacterium would be a negative or positive and determined the microscopic morphology (shape, and arrangement) of my bacterium as well. The test
Experiment 5- Limiting and Excess Reagents Purpose The purpose of this experiment was to calculate product and reactant amounts using reaction stoichiometry, as well as calculating the limiting and excess reagent in a copper (II) nitrate and potassium iodide reaction. We tested the reaction with different amounts of Cu(NO3)2 and KI making duplicates of each, with a total of 4 test tubes per group. Procedure Found in lab manual starting on page 91. 1. Collect 4 small test tubes and weight them.