Mixed Solutions: If 1 mmole of glucose (180mg=1mOsm) and 1 mmole of NaCl (58mg=2mmOsm) are put into a beaker and distilled water added to make 1 liter, the osmolarity is 3 mOSm/L. OSMOSIS CALCULATIONS: 1. Calculate the number of grams of NaCl needed to prepare 100 mL of a 280mOsm.L Solution. (280mosm/l) *(1L/1000mL)*(100mm/1)*(29mg/1mosm)*(1g/1000mg)=0.812g 2. Calculate the number of grams of glucose needed to prepare 100mL of a 280 msm/L glucose solution.
Part B: A similar experiment was performed after the 15% NaI in acetone solution. This time, ten test tubes were marked and we put 2 mL of 1% ethanolic silver nitrate in each test tube. The same corresponding halide was added to the mixtures as the first part of the experiment. Once these halides were added, we observed to see which turned cloudy. The mixtures that didn’t turn cloudy were put into a hot water bath of about 100 C for about a min.
Sac #1 placed into beaker #1 with distilled water, sac #2 placed into beaker #2 with 40% glucose solution and so forth. 3. Before analyzing, we had to allow sacs to remain undisturbed in the beaker for 1 hour. 4. After 1 hour we boiled a beaker of water on the hot plate (for Benedict’s and AgNO3 test).
If the compound does not melted after 2 minutes put “no” in the table. Part B - Solubility in Ethanol 1. Obtain six test tubes and add 25ml of ethanol to each test tube. 2. Add a two-gram sample of each of the above compounds to each test tube.
Gently stir the resulting mixture for 30-60 seconds. * Step 4 –Place a suitable filter (cloth, filter paper or a fine sieve) over an empty glass, and secure it with the rubber band. Pour the mixture into the filter. If the mixture is too thick, stir in more of the extraction solution. Leave to filter for about 10 minutes.
In one of these beakers, 1 mL of HCl was added to the solution, making this the “Yellow” beaker. In one of the other two beakers, 1 mL of NaOH was added to the solution, making this the “Blue” beaker. In the last beaker, 1 mL of distilled water was added to the solution, making this the “Green” beaker. Three dry cuvettes were obtained and filled with the “Yellow,” “Blue,” and “Green” solutions respectively. One final cuvette was filled with only distilled water (i.e.
Measure the solution by right clicking on the beaker and choose pH meter *Then I had to measure the pH of 0.1 M sodium hydroxide Get a 100 mL beaker from the equipment menu Right click on the beaker, select chemicals, and add 50 mL of 0.1 M sodium hydroxide. Measure the solution by right clicking on the beaker and choose pH meter Part 2: *First add 35 ml of unknown acid a to 100 mL beaker. Select all chemicals from the toolbar in the chemicals section, choose unknown acid a. Put the volume at 35 mL in a new 100 mL beaker. *Then add two drops of phenolphthalein indicator to the beaker by right clicking, choosing indications, and adding 2 drops of phenolphthalein.
Once the buffer tablet has dissolved into the water, add 10 ml of starch solution. 3. After the starch solution has been added, add 10 ml of enzyme solution to the current solution, simultaneously start the timer and record at 1 minute intervals. 4. In addition, place iodine in the dropping tile in order to identify if starch is currently present.
Gravimetric Determination of Sulfate Purpose The purpose of this lab is to determine the percentage of sulfate in the hydrate by precipitating the sulfate as barium sulfate. Materials Filler paper Sodium sulfate Graduated cylinder Bunsen burner Watch glass Beakers (250 mL, 400 mL) Rubber bulb Graduated pipette Beaker tongs Funnel Filter Paper Sodium Sulfate Drying oven Wash bottle Stirring rod Silver nitrate Hydrochloric acid Distilled water Small test tube Procedures First, .4861 grams of sodium sulfate was placed into a clean 400mL beaker. Exactly 200mL of water and 1mL of HCl was added to the same beaker. A watch glass was placed on the beaker and the solution was heated using the Bunsen burner to a gentle boil. The watch glass was removed with the beaker tongs.
They were glucose, water, lemon juice, Coca Cola, egg batter, lemon-lime soda, diet lemon-lime soda, Kraft Italian dressing, skim milk and butter. The positive control was the glucose and the negative control was the water. Twenty drops of each sample of substance was added to the test tubes (in all there were 10 test tubes). After the solutions were added in their own test tube, 20 drops of Benedict’s reagent was added. Each individual test tube was placed in boiling water and removed after 5 minutes to test if