Procedure: 1. Fill a beaker two-thirds full of water and add approximately 20 drops of IKI. Write down the solution's color and record the mass of the bag. 2. Do an initial Benedict's test on the 15% glucose/1% starch and the beaker solutions for glucose by putting some of the solution and a roughly equal amount of blue Benedict's solution in a test tube, placing the test tube in boiling water for 90 seconds, and observing whether or not the solution changes color from blue.
Materials and Methods Part 1 For the cation elimination test first 10 drops of potassium, iron (III), zinc (II), copper (II), and cobalt (II) were added to 5 centrifuge tubes and the color was recorded. Then for the metal hydroxide test, 6 M NaOH was added drop wise till a precipitate was formed. Each solution except potassium formed a precipitate, so then 10 additional drops of NaOH were added to the remaining solutions. Tubes were cleaned with distilled water and 6 M HCL. Next was the ammonia test 10 drops of each metal solution were added to new centrifuge tubes and 15 M NH4OH was added until the solution changed color or a precipitate was formed.
Samantha Monette Lab Section E Wednesday 10:00-12:50 p.m. Bromination/Debromination of Cholesterol Abstract: The purpose of this experiment was to purify commercial cholesterol. The commercial cholesterol was brominated using bromine as the reagent. After the compound was cooled and vacuum filtrated, dibromocholesterol was obtained. To purify the dibromocholesterol, zinc was added as the reagent. After a series of washes to remove any impurities, the compound was dried and decanted, cholesterol was synthesized.
With these solutions, the students were able to test whether not the amylase continues to break down starch with the presence of the carb cutter. Amylase mimics the enzyme activity that occurs in the body upon starch. The carb-cutter acts as the diet pills. The I2KI was used to add color to the solution. It binds with starch so the more starch, the darker the solution was.
A tablespoon of each drink was poured in a cup and its glucose concentration was determined before and after the addition of invertase with glucose test strips that were dipped into the cups. Ten trials were performed on each experimental group. An ANOVA test was done to determine the significance of the data. Several T-Tests were also run between the experimental groups showing the significance in differences of the beverages’ average glucose concentrations. The results partially confirmed the hypothesis as Coke had a significantly higher average glucose concentration than orange juice and milk, but not significantly higher glucose concentration than Gatorade and apple juice, therefore supporting the statement that Coke, Gatorade, and Apple Juice theoretically get the same amount of glucose into the bloodstream.
Results Sheet, Experiment 13 Brand of Vinegar used:________________ Acetic Acid % from bottle label_____________ | Initial NaOH Reading(estimate to 0.1 mL) | Final NaOH Reading(estimate to 0.1 mL) | Volume NaOH used(Final – Initial) | Trial 1 | 9.5 | 1.3 | 8.2 | Trial 2 | 9.6 | 1.4 | 8.2 | Trial 3 | 9.7 | 1.4 | 8.3 | Average Volume of NaOH used | 8.2 | Calculations: Normality of the acetic acid: .82 Mass of acetic acid: 49.2 % acetic acid (divide the grams, above, by the volume you used to get g/L): 4.92 Answer questions A-G in the lab manual: A. What is the average % acetic acid in your vinegar sample? Standard vinegar is 5% acetic acid. How does your result compare with the standard? The average was 4.92 which is pretty close comparably to the standard.
Materials & Methods Materials: · Scale · 4 6” Dialysis Tubing · 4 Transfer Pipets · Sugar · Scissors · Rubber Bands · 4 Same-Sized Coffee Cups · 250ml Graduated Cylinder · Tape Measure · Sauce Pan · 3 600ml Containers · Plastic Covering · Spoon Methods: 1.) Place 4 6” pieces of dialysis tubing into coffee cups full of tap with and leave them for two hours prior to beginning the experiment. 2.) As you wait, prepare your three sugar solutions. For the first solution, pour 5 grams of sugar into 250ml graduated cylinder and add water up to the 250ml mark.
EXPERIMENT ( 3 ) Topic : The preparation of Phenyl Benzoate from benzoyl chloride Introduction : The preparation of phenyl benzoate (ester) from phenol and benzoyl chloride is an esterification. In this experiment, you are requested to prepare and purify phenyl benzoate by some techniques. Chemicals : Phenol, Benzoyl chloride, Dilute NaOH, Methanol Procedures : 1. Dissolve 1 g phenol in 15 cm3 dilute NaOH using a 50 cm3 quickfit flask. 2.
b) What is the molar concentration (molarity) of ethanol in the wine? Chem 131 Solution Stoichiometry and Gas Law Problems Page 1 Problem 3 As part of some research for the product Alka-Seltzer, a researcher combines 0.840g sodium bicarbonate with 100.0 mL of 0.0500 M citric acid (H3C6H5O7). a) Write the molecular, complete ionic, and net ionic equations for this reaction (assume that each molecule of citric acid is completely neutralized). b) If the dry gas that the student actually collects occupies a volume of 0.196 L at 25°C and 1.00 atm pressure, what is the percentage yield of this reaction? c) The researcher
Experiment 2 Time | Observations | 5 minutes | Bubbled like sugar | Once salt water was added | Turned soapy white and thick | Equation: METHOD 1) Put 2 cm3 of castor oil into a 250 cm3 beaker and add 10 cm3 of 5mol.dm-3 sodium hydroxide from a measuring cylinder. (Take care when measuring this out and adding it to the beaker to avoid any drips and spills.) 2) Heat the mixture gently over a tripod and gauze, STIRRING CONSTANTLY with a glass rod (otherwise the mixture erupts). Allow to simmer (boil gently) for 5 minutes. Steady the beaker using