Do an initial Benedict's test on the 15% glucose/1% starch and the beaker solutions for glucose by putting some of the solution and a roughly equal amount of blue Benedict's solution in a test tube, placing the test tube in boiling water for 90 seconds, and observing whether or not the solution changes color from blue. 3. Form a bag out of dialysis tubing by tying off one end, putting in enough 15% glucose/1% starch solution to fill it halfway, and tying off the other end leaving the other half of the bag void of anything (even air). Write down the solution's color. 4.
Cook for 4 more minutes, then drain liquid from pot and pour contents over a clean, unused large garbage bag or newspapers. Serve with melted butter and enjoy!! Let the water boil in a pot that is 3/4 full with two seasoning packets and 2-4 cut up lemons. Add 2 bottles of Newman’s
Pour of most of the liquid making sure not to pour away any of the solid at the bottom of the container. Add about 10 mL of water, swirl the solution, and again pour off most of the liquid. Record your observations. 15. Clean up your area and return equipment.
We now slowly pour the solution into a funnel with filter paper. The extract along with 2 pieces of Iodine are added to a new beaker and left for 10 minutes. Finally to determine if the lab was successful, three tests are conducted for Iodine, Iodide and triiodide. The objective is to produce a tincture of iodine by extracting iodide and other components from seaweed. Warm up Activity 1.
Experiment 2 Time | Observations | 5 minutes | Bubbled like sugar | Once salt water was added | Turned soapy white and thick | Equation: METHOD 1) Put 2 cm3 of castor oil into a 250 cm3 beaker and add 10 cm3 of 5mol.dm-3 sodium hydroxide from a measuring cylinder. (Take care when measuring this out and adding it to the beaker to avoid any drips and spills.) 2) Heat the mixture gently over a tripod and gauze, STIRRING CONSTANTLY with a glass rod (otherwise the mixture erupts). Allow to simmer (boil gently) for 5 minutes. Steady the beaker using
I believe the household items will be on the extremes, either extremely basic or extremely acidic. Methods Items for experiment were purchased from the Commissary and the Nhetto. They include: 1 x .608 kg head of red Cabbage 2 x Melitta white coffee filters 1 x 12 oz coke 1x Tums antacid 1x Tilex Mold and Mildew Remover 1x 22oz bottle of Clorax bleach 1x bx of Arm and Hammer Baking Soda 1x toilet bowl cleaner 1x hohes C orange juice 750ml of tap water The following steps from the guide was followed (with changes from the instructor): 1. Using a standard cheese grater, I took the entire head of red cabbage and shredded it into small pieces. After shredding was complete the pile of cabbage was placed into a stainless steel cook pot.
The liquid of homogenate was filtered into a beaker through Miracloth (2 layers cloth) to remove large plant components and 1 ml of the filtrate was transferred to a conical tube. 8.4 g of ammonium sulfate was slowly added to the 40 ml of the filtrate as it was stirred on a stir plate for 15 min to achieve 37% saturation (210g/L of solution). The solution was then centrifuged at a speed of 9000 x g at 4oC for 15 min to sediment the proteins. The resultant supernatant 1 was transferred to a beaker with 1 ml transferred to a conical tube and the obtained pellet 1 was resuspended in 4 ml of distilled water and transferred into a dialysis bag to remove the salt. Then, 3.4 g of ammonium sulfate was slowly added to the supernatant 1 as it was stirred for 15 min to achieve 50% saturation (85g/L of solution).
Use your 4 markers to draw a different colored dot on each of the pencil marks on the paper strip. Allow the markers ink to dry, and then go back recolor each dot. 5. Prepare the saltwater mixture by mixing 1/8 teaspoon of salt and three cups of water in a clean 2-liter bottle. Stir or shake the solution until it is dissolved.
Bring the contents of the distillation flask to a gentle reflux (i.e., vapors are condensing in the first ¼ to 1/3 of the vertical condenser and dripping constantly back into the flask below). Maintain this reflux for 10 minutes, then increase the heat in order to distill the liquid into the collection flask, then the column, and even the distillation head with aluminum foil (shiny side IN). Continue distillation of the cyclohexene product until the volume in the distillation flask is reduced to about 1 mL. Ask the instructor to assist you in removing the heat source and sand bath for the almost empty flask; use Ove Gloves and/or clamps to avoid burns. |
Then proceed to decolorize with 95% ethanol until the ethanol running off the slide is clear. Briefly rinse with distilled water. Counter-stain with safranin (secondary stain) for 1 minute then rinse with distilled water and gently blot dry with a paper towel. Lastly observe slide using oil immersion with the 100x