Dna Extraction Essay

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DNA Extraction Aim: To extract DNA from a carrot using house hold products. Method: * Step 1 – Pour 60 ml (¼ cup) of clear alcohol into a glass. Place the glass into the bowl of iced water to chill, then set aside. * Step 2 – Pour 120 ml (½ cup) of tap/distilled water into the measuring cup with the salt. Add 30 ml (2 tablespoons) of clear detergent and mix carefully until the salt is dissolved. This is the extraction solution. * Step 3 – Grate a large carrot or 2 medium sized carrots then add some of the extraction solution or blend the carrot with salt solution, then add the detergent. The consistency should be similar to a thick fruit smoothie. Gently stir the resulting mixture for 30-60 seconds. * Step 4 –Place a suitable filter (cloth, filter paper or a fine sieve) over an empty glass, and secure it with the rubber band. Pour the mixture into the filter. If the mixture is too thick, stir in more of the extraction solution. Leave to filter for about 10 minutes. * Step 5 – Some liquid should have filtered through (the filtrate). Remove the glass of alcohol from the iced water. Gently pour the alcohol along the wall of the glass containing the filtrate. Observe after at least 5 minutes. For a closer examination, use the glass rod, popsicle stick or toothpick to hook the precipitate. * Step 6 – Pour the liquids down the sink and clean the rest of the equipment. Read more at Suite101: How to Extract DNA From Fruit and Vegetables: A Home Experiment for Viewing Real Deoxyribonkucleic Acid http://www.suite101.com/content/how-to-extract-dna-from-fruit-and-vegetables-a76806#ixzz1EkVq2LaJ Theory The mashing breaks the cells apart. The detergent breaks them open, freeing the cell contents (including the DNA). The filtering removes most of the unwanted larger particles. The salt has positively charged ions, which stop the negatively charged DNA

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