How Does Sucrose Concentration Affect Yeast

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Minutes 0% Start 5 10 15 20 25 30 35 40 Trial 1 3 3 3.1 2.6 3 Table 1 Trial 2 4 3.5 3.5 3.5 3.5 Trial 3 0.5 3.5 3.6 3.6 3.5 Any source of error would include a lack of time management. Minutes Table 2 2.5% Start 5 10 15 20 25 30 35 40 Any source of error would include a wrong amount of sucrose or yeast. Trial 1 3 3.4 4 3.2 Trial 2 3 3.1 3.1 3.1 Trial 3 2.5 3.4 3 3.7 Minutes 5% Start 5 10 15 20 25 30 35 40 Trial 1 3.4 3.8 3.9 Table 3 Trial 2 3 4.3 3.4 Trial 3 2.8 3.5 3.5 Any source of error would include time management as well. Minutes 7.5% Start 5 10 15 20 25 Any source of error would include the lack of timing the experiment properly. Minutes Table 5 10% Start 5 10 15 Trial 1 4 3 Trial 2 3 4 Trial 3 3.5 3 Trial 1 3 3 3 Table 4…show more content…
We waited 5 minutes for the respirometers to equilibrate after we measured the yeast solution in the graduated cylinder. I found that giving the conclusive data by recording the amount of milliliters in the pipette left after the 5 minutes were up. We had three trials all with 2.5% , 5% , 7.5% , and 10% solution. Limitations of Experimental Design Some possible reasons for error is the percentage of sucrose we contained in each trial of the experiment. This would affect the volume of gas captured in the pipette. And with a limited amount of sucrose its possible sucrose wouldnt properly fill in as a source of glucose for cellular respiration. Another source of error would be that we didnt let the burner heat up all the way to the correct temperature so that would affect the outcome massively and significantly. Also when we had 10% sucrose, we only recorded the time two times, compared to the other percentages of sucrose where we had many other trials and times recorded. We also found problems when we had placed clay on the tip of the pipette to seal the air out. On some of the pipettes the clay was not properly sealing the pipette causing it to leak. Suggestions for Improvement Some suggestions for improvement would include to properly place the clay correctly sealing the 5mL pipettes. Also, for the temperature recordings, the lab could of been improved by letting the burner heat up to the proper temperature and then placing the pipette into the

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