Characteristics Of The Aldox Gene

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Genevieve 171L Honors 11/6/08 Characteristics of the Aldox Gene Introduction This experiment was conducted by using aldehyde oxidase (AO) to genetically characterize the aldox gene. It was hypothesized that the aldox gene is recessive and sex linked. Fruit flies (Drosophilia melanogaster) are genetically well known eukaryotic organisms that were used to investigate the inheritance of the aldox gene. To determine if AO is present, an enzyme spot test was used. The yellow color indicated that AO was present and active while blue color indicated AO is not present. Data Table 1 Enzyme Assay F1 | | Male | Female | Blue | 2 | 3 | Yellow | 8 | 7 | Table 2 Enzyme Assay F2 | | Male | Female | Blue | 3 | 3 | Yellow | 9 | 9 | Figure 1. F1 Generation A A Aa | Aa | Aa | Aa | a a Figure 2. F2 Generation A a AA | Aa | Aa | aa | A a Figure 3. Sex-Linked X+ Y X-X+ | XY | X-X+ | XY | X- X- Of the ten females in the F1 generation, three were negative and seven were positive for the AO enzyme. The ten males tested had two negative and eight were positive flies for the AO enzyme. The F2 generation, of the twelve females and twelve males, three were negative and nine were positive for the AO enzyme. The crossing in the F1 generation of a dominant male (AA) and a mutant female (aa) developed heterozygous-dominant offspring. The crossing in the F2 generation of the heterozygous male and heterozygous female produced two heterozygous offspring, one homozygous recessive offspring and one homozygous dominant offspring. Discussion This experiment was conducted by using aldehyde oxidase (AO) to genetically characterize the aldox gene. It was hypothesized that the gene

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