Study of Krebs Cycle Using Mitochondria from Mung Bean Seedlings

2017 Words9 Pages
Introduction: The Krebs cycle is a central metabolic pathway that is responsible in producing most of the energy, required for living for most organisms, in the form of ATP. It produces FADH2 and NADH which are used by the electron transport chain (oxidative phosphorylation) in the mitochondria to produce ATP. The Kerb cycle takes place in the mitochondria. This is the reason that mitochondria are known as the energy centers in our cells. This energy is used to make the cells function. A dysfunction of mitochondria is known to result in diseases that cause build-up of Lactic acid in the body and many other problems. This is why it is important to study the mitochondria, to understand how the Kerb cycle works in producing ATP which can help us better understand the role of the mitochondria (Chariot et al. 1999). In this experiment the mitochondria will be studied using in vitro methods. The Mitochondria will be extracted using a Centrifuge and Rapid Homogenization from an etiolated Mung bean seedling. The interaction of the mitochondria to different chemical conditions will be studied to determine how the Kerb cycle and oxidative phosphorylation function. The chemical condition will include the effects of the presence and absence of Na azide, Na succinate and malonate on the mitochondria. This will be tested using a 2, 6 dichlorophenolindophenol (DCPIP) dye which is coloured in its oxidized form but when it is reduced it will become colourless. The reduction happens when the Krebs cycle produces reducing agents for the reduction to happen. This will help us identify and compare the rate at which these reducing agents are formed under the different condition (Carleton University. 2012). The objective of this lab is to extract mitochondria from an etiolated Mung bean seedling in order to study the metabolic functions of the mitochondria. Writtin description: Table

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