Biolfilm Case Study

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Biofilm Case Study 1. Legionnaire’s Disease is caused by the bacterium called legionella. The bacteria grow best in warm water, like the kind found in hot tubs, cooling towers, hot water tanks, large plumbing systems, or parts of the air-conditioning systems of large buildings. Legionella live within amoebae in the natural environment. This is a problem on cruise ships in the whirl pools, hot tubs, and cooling systems if they do not treat these with the proper chemicals to prevent this bacterium from growing and it does anyone can breathe in a water droplet and possibly spread it to another person. 2. (A) The Direct Fluorescent Antibody Test is used to detect the presences of a specific antigen (or a specific protein on the surface of a virus, bacterium, or other microbes). This test works by fluorescent chemicals attaching to the constant region of an antibody. If the antigen (legionella) is present the antibody binds to generate a very specific protein tag. (B) None of the water samples tested positive for legionella. For the swabbed samples the hot tub was the one that tested positive for the bacterium. In all of the samples the hot tub sample was very bright, highlighted the bacterium whereas the other samples didn’t have the same appearance. 3. We set up a prototype water system. It consisted of a series of square PVC pipes with 24 removable pieces on one side. Water flows in one end and out the other end. We controlled the flow rate, and the content of the water that flows through each pipe. Based on my experiment here is what I found… | Day 0 | Day 10 | Day 25 | Control | ++ | +++ | ++++ | Chlorine | ++ | +++ | ++++ | Heat +Chlorine | ++ | ++ | +++ | UV Radiation | ++ | +++ | ++++ | Ozone | ++ | ++ | ++++ | Ozone + Chlorine | ++ | +_ | +++ | 4.) The ozone and chlorine would be the treatment I would recommend. Based off the
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