Leave to filter for about 10 minutes. * Step 5 – Some liquid should have filtered through (the filtrate). Remove the glass of alcohol from the iced water. Gently pour the alcohol along the wall of the glass containing the filtrate. Observe after at least 5 minutes.
12 Mung Beans (6 per bottle) 10 Ml of water per bottle per day. A ruler to measure your plants each day. (cm) A data table to collect all the data you gather over your 10 day time period. Procedure: Follow the following steps: Step 1- Get 2 clear 2 liter bottles. Step 2- Clean out both 2 liter bottles for any excess soda, water, etc.
Allow the mixture to cool for a few minutes then filter it, using either gravity or vacuum filtration. (We shall be using vacuum filtration.) Wash the residue in the funnel once with a little water and collect all the filtrate. 4. Pour all the filtrate and washings into a 250cm3 volumetric flask.
Add 0.2 ml of yeast invertase and incubate at the same temperature for 6 mins. After incubation quench with 8.0 ml of 0.1M H2SO4 and mix thoroughly. Transfer 0.1ml aliquot of sample to a separate test tube and add 0.9 ml of H2O followed by 4ml of WSG and incubate at afore mentioned temperature for a period of 15 mins. The mixture is then assayed at 500nm. Procedure is to be repeated using temperature of 0o, 60o, and 80o C. Introduction Enzymes are single-chain or multiple chain proteins that act as biological catalysts with the inherent ability to promote specific chemical reactions in vivo, as well as in vitro.
The watch glass was removed with the beaker tongs. Using a rubber bulb and a stirring rod to stir the solution continuously, 15.00mL of .25M BaCl2 solution was added to the solution in the beaker. The watch glass is replaced and the solution is keep hot but not boiling for 15 minutes. The precipitate was allowed to settle. When the liquid above the precipitate was clear, the solution was tested for completeness of precipitation when a few drops of BaCl2 solution were added from a pipette.
After heating, the mixture was cooled to room temperature and filtered by vacuum filtration into a fritted funnel to yield a purple product. The product was washed 3 times with (5mL) portions of chilled 6M HCL, then Ethanol, and lastly with acetone. The resulting product was placed into a vial and left to dry in a vacuum desiccator for 1 week and weighed the next week. The yield was 6.029g. The second experiment, procedure 1, combined [Co(NH3)5 (H2O)]Cl2 (0.0060M, 1.52g) and (25mL) of distilled water to an 125mL Erlenmeyer flask.
The liquid of homogenate was filtered into a beaker through Miracloth (2 layers cloth) to remove large plant components and 1 ml of the filtrate was transferred to a conical tube. 8.4 g of ammonium sulfate was slowly added to the 40 ml of the filtrate as it was stirred on a stir plate for 15 min to achieve 37% saturation (210g/L of solution). The solution was then centrifuged at a speed of 9000 x g at 4oC for 15 min to sediment the proteins. The resultant supernatant 1 was transferred to a beaker with 1 ml transferred to a conical tube and the obtained pellet 1 was resuspended in 4 ml of distilled water and transferred into a dialysis bag to remove the salt. Then, 3.4 g of ammonium sulfate was slowly added to the supernatant 1 as it was stirred for 15 min to achieve 50% saturation (85g/L of solution).
We then shook the jar until it was well mixed and waited about thirty minutes for the mixture to settle. Once it settled we measured the thickness of each layer of the soil. In the water holding experiment we simply filled a beaker with 100 ml. with garden soil then poured water into the beaker to saturate the soil enough so that there was no air trapped in the dirt. We measured the amount of water we used and calculated the pore space as well as the percentage of pore space.