First, 0.4040 grams of NaH2PO4 and 0.3989 grams of Na2HPO4 were added to a 150 mL beaker, along with 50 mL of distilled water. Next, 20 drops of bromothymol blue were inserted into the solution. The program LoggerPro and a Vernier pH probe were used to calculate the pH. Then, a volumetric flask was used to measure 5 mL of the buffer solution into each of three, separate 50 mL beakers. After rinsing a volumetric pipet with 1.0 M HCl, 1.00 mL of 1.0 M HCl was transferred to one of the three beakers.
The buffer is removed and rinsed with the electrodes with distilled water. It is then dipped in acetic acid and the pH is recorded. A burette was set up to add the NaOH. 1mL of NaOH is added. The solution is swirled and the pH is measured.
The water then became saturated. 4. The cold beaker was Beaker A. After Beaker A was tested, Beaker B was tested, which had been sitting on a heater keeping the water inside warm at 30 degrees Celsius. It was filled with KCI five times by the 5g spoon, and then five times again by the 1g spoon.
I added a few drops of 3% Hydrogen Peroxide and it resulted in bubble formation. The positive gram stain and positive catalase test indicated that the organism belonged to the Staphylococcus Genus, which meant it was now time to pinpoint the species based on differential and selective biochemical tests. To ensure that my genus identification was correct I performed a Bile Esculin Slant with a fish tail streak on my unknown bacteria. This test resulted in neither growth nor a black precipitate; therefore I could conclude that my unknown was not Streptococci or Enterococci but indeed Staphylococcus. Next I observed the isolation streak on my Blood Agar Plate and found pinpoint, round, entire and flat colony morphology as well as an alpha hemolytic reaction pattern, indicating red blood cell ion leakage which is characteristic of S. epidermidis.
4. Make sure you set the data collection system to Absorbance at wavelength = 635 nm. 5. Measure 100 mL of distilled water into a 250 mL beaker. Add two drops of food coloring to the beaker of distilled water and mix thoroughly.
What should this contain? j Count up how many iodine drops you have used, each one equals 10 seconds of reaction time. k Repeat the whole procedure with another of the pH buffers or pool your results with others in your class. l Collect repeat data if there is time. m Plot a graph of time taken for starch to break down against pH.
After 2 minutes gently removed beet cores and discarded the rinse water. 3. Placed each beet core into each of five clean dry test tubes. 4. Labeled each test tube with the solvent treatment that it was to
Place micropipette #1 on the laboratory balance. Measure its mass to the nearest 0.01 g and record this value in Data Table 1. 3. Fill the micropipette with tap water as completely as possible. Place it on the balance and measure its mass to the nearest 0.01 g. record the mass in Data Table 1.
• Fourth, clamp the other end of tubing, leaving about a 2 cm. pocket of air. • Fifth, label the tubing with a letter of solution in bag. • Sixth, rinse each bag with water then blot dry with a paper towel. • Seventh, measure the mass of each bag and record data.
6. Place soiled clothes in a plastic bag. 7. Wash child’s hands under running water with soap and dry their hands with a disposable towel. 8.