Your answer: yes;results showed they have partial identity Activity 3: Indirect Enzyme-Linked Immunosorbent Assay (ELISA) Lab Report 1. Describe how the direct and indirect ELISA are different. Your answer: Direct ELISA is directly looking for the foreign substance. The microtiter plate is coated with homologous antibodies made against the antigen of interest If the antigen is present, a sandwich of antibody, antigen and secondary antibody will form Indirect ELISA is designed to detect antibodies that the patient has made against the antigen.The microtiter plate is coated with
Emilio Lajara Pre-Lab Write Up Chapter Three 02-05-08 Objective- Proteins are made up of amino acids. All amino acids are found in cells. They have an acidic group and an amino group. A peptide is when one or more amino acids form a chain, while a polypeptide is a long chain of amino acids. A protein can contain one or more polypeptide chains.
1-2-09 Glowing Bacteria Genes are pieces of DNA which hold the instructions for making proteins. The protein will then give an organism a particular trait. Genetic transformation means changes caused by genes, and requires the insertion of a gene into an organism, in order to change the trait. this technique is used by in many areas of biotechnology. Agriculturally, plants can be genetically transformed to be resistant to pesticides, spoilage, and frost.
Unknown Lab Report: Enterobacter cloacae Jessica Sainvil Professor Cupido Bio 253L1 Thu 9:30-12:30 Due Date: 12/6/12 Unknown#103 Introduction The bacterium Enterobacter cloacae isolated from the given #103 was a Gram-negative Rod. The purpose of this lab was to isolate and identify the genus and species of an unknown bacterium. It is important to identify an unknown microorganism because knowing how the bacteria work and how it is structured means knowing how it can affect humans. Unknown bacteria may also be used clinically many pharmaceutical drugs are based on products made by organisms (Katzung, B.G.2004). In order to identify the unknown organism a series of tests were performed.
There is free rotation around this group because resonance is limited to resist the free rotation. 5) What is a Ramachandran plot and how does it relate to bond rotation in peptides? Ramachandran plot is a visualization method of dihedral angles φ against ψ of amino acid residues in protein structure. Overall, it provides the different confirmation possibilities of the two. 6) Which amino acid has more restrictive bond rotation and why?
It selectively filter ions and organic molecules; controls the movements of substances in and out if the cell. 3) In what type(s) of organisms would the Gram stain not work? Why? - Because Gram staining process target specific on cells that has peptidoglycan, cell membrane, and cell wall so organisms that doesn’t have such will not be detected by Gram staining process, organisms such as virus. 4) How is the Gram stain reaction by bacteria useful information to medical doctors or microbiologists?
According to Rebecca Tuhus-Dubrow, Pre-implantation genetic diagnosis (PGD) targets severe childhood diseases, such as Tay-Sachs, sickle cell anemia, and colon cancer. Hence it reduces the chances of lifelong health issues. Linkage mapping provides an important application in forensic science. The laboratory techniques to prevent inherited diseases show an important impact in forensic applications. The forensic application of genetic mapping helps in the
Gel Filtration of Proteins Session 4 Lab Report Semester 2 Victoria Franks 2930499 Gel Filtration of Proteins Introduction The aim of this experiment was to separate a range of proteins from a single sample, using their differing sizes to tell which protein was which when passed through a gel barrier. This method is called size exclusion chromatography. A range of coloured proteins and dyes are used so that samples can be easily idenitifed by the naked eye; however, two of the proteins are a similar colour, making it necessary to use spectrophotometry to tell the difference between them, based on the fact that they have different levels of absorbancy. A gel called Sephadex is used within a tight column and the protein sample is passed through using a strong buffer to help the smaller molecules of the protein sample to diffuse into the gel. As the Sephadex forms a network with tiny holes, this prevents molecules that are too large from getting inside.
Experiment #2- Amino Acids October 16, 2013 Jessica Ramirez Kara Lue Chem 353- Section Instructor: Professor G. Santillan Purpose The purpose of this experiment is to become familiar with the dual nature of amino acids and their unique ability to be electrically neutral, or zwitterion. When amino acids sit in the blood stream they act as proton acceptors or donors, and this change in pH affects the overall charge of the amino acid. The titration curve is used to determine an amino acid through estimated pKa values of the ionizable groups of the amino acid. Knowing the pI helps us understand the acid-base behavior of an amino acid. Principles Amino Acids are weak polyprotic acids, which means contain more than one mole ionizable hydronium ions per mole of acid.
How the structure of proteins is related to their function Essay The primary structure of a protein is made from long chains of amino acids joined by polypeptide bonds. Its secondary structure is when the amino acid sequence starts to coil or fold into either a beta pleated sheet or an alpha helix. The tertiary structure determines the shape of a proteins active site. The tertiary structure is made up of hydrogen, ionic and disulphide bonds between the amino acid sequences. If the amino acid sequence is altered in any way it will have repucutions on the secondary and tertiary structure because bonds will be formed in different locations and therefore a different 3D structure will be present, thus meaning shape of the active site will change in enzymes.