In determining this unknown bacteria, number two, many tests were performed. The tests that helped identify this bacteria were gram-stain, motility, Simmons citrate, lactose testing, and methyl red testing. All of the tests that were stated and recorded on the comparative analysis chart also tribute to uncovering the unknown bacteria, but they were not the critical tests needed. In the gram-stain tests, the bacteria was indicated as negative and the shape of the bacteria were rods. Motility was noted under the microscope by evidence of the bacteria moving around on the slide.
In Microbiology Lab 3 I chose the unknown culture #14 and ultimately identified it as Staphylococcus epidermidis bacteria. On a microscopic level I found the organism to be gram positive with a coccobacillus shape and both tetrad and cluster cell arrangement. I performed an isolation streak with S. epidermidis on Nutrient Agar which resulted in pinpoint, round, entire, and flat macroscopic morphology. I took a loopful of the organism from the Nutrient Agar and placed it on a slide to perform the catalase test. I added a few drops of 3% Hydrogen Peroxide and it resulted in bubble formation.
It is for this reason that Identification of bacteria is very important in the many areas of microbiology (Singh, 2009). There are many reasons for identifying an unknown bacterium. The reasons range from medical purposes, such as determining if the unknown could cause ailments in living things or knowing what microorganisms are needed to make antibiotics to other purposes such as knowing the exact microorganism has to be used to make certain foods (Burton, 2003). Throughout this report we will be addressing the differences in methods of identifying unknown microorganisms and differentiate between the results of these techniques by explaining how each outcome was achieved. The main aim of these experiments is to determine whether the unknown microorganisms are of Gram-Positive or Gram Negative nature and ultimately to determine exactly what kind on microorganism the unknowns are.
Calonna Taylor Professor Osborne BSC2010C 9 February 2014 Beyond The Cells Purpose: The purpose of this lab report is to become familiar with the use of a compound microscope and the Gram Stain. Hypothesis: If you do not know how to use a Microscope or how to Gram Stain, then your results of your lab will come out inconclusive or just wrong. Part A: Cell Structures Materials & Methods: Materials: * Colored Pencils * Paper * Access to a scanner Methods: 1. View video “Using a Microscope.” 2. Look at the slides and identify the cell structure within.
Unknown Laboratory Report Identification of Unknown # 15 Ashley Grace C. May 4th 2011 Elizabeth Russo Microbiology 274 Spring 2011 INTRODUCTION There are many reasons behind the importance of identifying unknown bacteria. Reasons range from finding out how it can impact humans and the cause of a specific infection and diseases, to research for potential new pharmaceuticals to cure diseases. It is also important as bacteria are always mutating and may identify new strains that cause diseases or identify microbes that have become resistant to antibiotics. We can determine an unknown microbe by performing a variety of physiological and biochemical tests. MATERIALS AND METHODS Materials and methods are explained in chronological order.
The nutrient agar was incubated at 37 degrees Celsius for 48 hours. Upon observation on day two the nutrient agar plate appeared to be tinted a light green color, which is comparable to the pigment of the agar completed in exercise #10 on the lab manual, which led to the belief that the gram negative bacteria was Pseudomonas aeruginosa. To confirm these beliefs, a new isolation streak of organism A’s pure colony was created. On day three a gram stain of the pure colony was completed following the steps for gram stain on page 67 of the lab
In this experiment there were main 4 hypothesis made according to each agar plate each with a different types of genes on them. There are two genes that are on the agar plate and not in the solution it is the ampicillin and the arabinose genes. The ampicillin gene (amp) is a gene which acts like an antibiotic that kill the bacteria but when used with the pGLO plasmid it lets it survive because of the ampicillin resistant. The arabinose
The purpose of these tests were to determine how the bacterium reacted to glucose with and without oxygen. My findings reported a gram stain negative along with positive tests for glucose oxidation and fermentation. Next I performed an Indole Production Test. This test determines whether the microbe produces indole from the amino acid tryptophan. The results from this test were negative.
MEMO To: Carrie Johnson From: Staff Biologist RE: Bacteria The first step in identifying the mystery organism is to know what kingdom it belongs to. It will belong to one of the following kingdoms: animals, plants, archaebacteria, eubacteria, fungi, or protista. Organisms are placed into these categories based on similarities or common characteristics. Some of the characteristics that are used to determine placement are cell type, metabolism, and reproduction. Examine your living organism and determine if it is a bacteria, achaean or a eukaryote.
|Staphylococcus Aureus in a clinical setting | | | |Microbiology Laboratory, Sierra College, Rocklin, CA 95677 | | A culture taken from a clinical setting was grown and isolated expected to grow Staphylococcus aureus due to the high numbers of | |infections. The pure isolated sample is a Gram positive Staphylococcus that was identified as Staphylococcus auricularis by automated| |nucleic acid sequencing. A series of enzymatic test were run with abnormal results not matching proven data. The failed hypothesis | |revealed an organism that may be utilized as a waste water treatment product. | | | INTRODUCTION In the clinical setting there are many microorganisms thriving.