Put the stopper in the flask in order to drive out all the air and any excess water. Work the stopper gently into the flask, so that it is firmly seated in position. Wipe any water from the outside of the flask with a towel and soak up all excess water from around the top of the stopper. Again weigh the flask to the nearest milligram. Having the density of water and the temperature of water, you should be able to determine the volume of the flask very precisely.
The column is now ready for the addition of the mixture to be separated. 2.) Separation of the Fluorene and 9-Fluorenone Mixture: Accurately weigh 0.1g of the 1:1 mixture of fluorene and 9-fluorenone mixture, place it in a small test tube, and dissolve it in 0.5mL of petroleum ether. Add a few drops of dichloromethane, just enough to effect solution. Transfer the solution to the top of the column with a Pasteur pipet.
II. Procedure Use a 10-mL graduated cylinder to put 6mL of 6 M sodium hydroxide solution (NaOH) into a clean 500mL storage bottle. Use a 100mL graduated cylinder to add 345mL of distilled water to the plastic bottle. Place a lid on the bottle and mix by shaking repeatedly. Sit the bottle off to the side for later use.
Add a drop of water using and inoculating loop to a clean glass slide. Then using a sterilized loop, smear the slide with the organism, let the slide dry completely and heat fix. Apply crystal violet stain (primary stain) for one minute, briefly rinse with distilled water. Apply iodine (mordant) for one 1 minute, briefly rinse with distilled water. Then proceed to decolorize with 95% ethanol until the ethanol running off the slide is clear.
Lab Report: Crystallization of phthalic acid from water (microscale) Abstract The 0.60 g of phthalic acid is put in a water solution, which is brought to a boil to dissolve the acid into water. After the solution was clear with just the rock visible the test tube solution is now cooled in ice and stirred till crystals appear. Excess water is then removed, then the adding of ethanol is done to separate the water from the crystals. Tube is then placed in a beaker of hot water which aids in the removal of the solvent. After drying the compound the percent recovery was found to be 70%.
Allow the precipitate to settle, and heat 30 mL distilled water. Weigh, fold and place two filter papers into funnels and seal with distilled water. Decant a 50 mL sample from each beaker of solution into two other beakers. Pour the rest of the solutions into the respective funnel filters, and make sure to wash all precipitate into the filter, using the heated distilled water. Remove and air dry the filter papers containing the precipitates.
5a. Place a small amount (the size of an aspirin tablet) of copper II sulfate pentahydrate in a clean dry test tube and record your observations. Using your test tube holder, tilt the test tube and gently heat the contents for 4-5 minutes, while recording your observations. Make sure to observe the upper end of the test tube while heating. Allow the test tube to cool and record your observations.
Write down everything you notice. Remove test tube from water and allow it to cool for 2 minutes in a small beaker of tap water. Add about 7.0 mL of 1M hydrochloric acid to the tube. Mix chemicals very thoroughly with a stirring rod. Record how long it takes for change to happen.
Bring the contents of the distillation flask to a gentle reflux (i.e., vapors are condensing in the first ¼ to 1/3 of the vertical condenser and dripping constantly back into the flask below). Maintain this reflux for 10 minutes, then increase the heat in order to distill the liquid into the collection flask, then the column, and even the distillation head with aluminum foil (shiny side IN). Continue distillation of the cyclohexene product until the volume in the distillation flask is reduced to about 1 mL. Ask the instructor to assist you in removing the heat source and sand bath for the almost empty flask; use Ove Gloves and/or clamps to avoid burns. |