Lab Report Ph Buffer

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I. Objective The purpose of this experiment was to define and understand the meaning of the following terms: acid, base, pH, pH scale, pH indicators, buffer system, acidosis, and alkalosis. To determine the acidic or basic nature and actual pH of various substances. To discuss the formation of the carbonic acid/bicarbonate ion buffer system in the blood. To understand the important role of the carbonic acid/bicarbonate ion buffer system in maintaining homeostasis, especially with regard to ventilation. Experiment was to show how the buffering agent minimized the change in pH (WCCC,2014) II. Introduction (Background info.) Homeostatsis is the maintenance of a relatively stable internal ennvironment. For Cells to survive, the composition of the surrounding fluids must remain relatively stable. If cells survive, the individual survives. The ECF (extracelluar fluids) is the fluid outside the cells. The ECF mediates the exchange of materials between the external environment and the cells of the body. EFC consist of the following compartments: Interstitial or tissue fluid, plasma and transcellular fluids. Three conditions must be met in order for homeostatis to occur: 1. Must contain an optimum concentration of gases, ions, nutrients and water. 2. Must maintian an optimum temperature and 3. Must maintain the optimum pressure(volume) for the health of the cells. III. Materials Part A. Utilization of Indicator Strips 4 Red Litmus indicator paper 4 Blue Litmus indicator paper 50 ml beakers Added 2 drops each of: vinegar, ammonia, hydrochloric acid(HCL), and sodium hydroxide (NaOH) (Materials continue) Part B. pH, Buffer determination Orion Model 420 pH meter 0.1m HCL Distill water 0.1m NaoH 7.0 buffer solution 5 150ml beakers Part C. Respiratory

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