A. Nicotiamide adenine dinucleotide B. Flavin adenine dinucleotide C. Thiamin pyrophospate D. All of the above E. A and B 4. How does a riboflavin deficiency affect vitamin B6 metabolism? A. Riboflavin, as its coenzyme form flavin mononucleotide, is associated with an oxidase responsible for converting pyridoxine phosphate or pyridoxamine phosphate to pyridoxal phosphate. B. Riboflavin is the coenzyme form that is associated with an oxidase responsible for converting pyridoxine phosphate or pyridoxamine phosphate to pyridoxal phosphate. C. Dephosphorylation of the vitamin B6 vitamers during digestion requires flavin mononucleotide.
Protein Chromatography LAB Introduction: The basic method of separation of biomolecules is based on their physicochemical characteristics such as; polarity (solubility, volatility, and adsorption) size/mass (diffusion, sedimentation) (Boyer, 2009). In this experiment include separating a mixture of protein mainly the four below on the basis of their size through a process known as size exclusion chromatography (gel filtration chromatography), and Gel Electrophoresis (SDS-PAGE) for size, charge and purification separation. In a gel filtration (also known as column chromatography) the column is tedious method to make however can be repeatedly used for several years after several washings samples are mostly separated as per molecular weight but the sensitivity is lower it is used in preparative methods i.e. in case one would want to separate a large amount of protein (or any other metabolite) so has to purify it so that it can later be used for some other experiments the sample gets very diluted and concentration of the sample port filtration is a must Gel Electrophoresis (SDS-PAGE ) is easy to prepare and is usually a onetime use and discard the samples can be separated by both charge and mass (by mass after negating the charge) it is used as an analytical method ie to check the presence/absence of a protein or gene u can only load small amounts of sample. Protein | M.W.
BIO 132 Digestive Physiology PRELAB questions What is the purpose of this laboratory? These experiments will examine the effects of enzymes, variable ph and temperature on ingested starch, fats, and proteins in the digestive system What are the products of digesting: Starch? Glucose Fats? Fatty acids and Glycerol Proteins? Amino Acids What is an enzyme and how does it work?
Scaled Beaker 4. Dropper 5. Acidic solution 6. Basic solution Hypothesis “Does change in pH affects an enzymatic activity”? Testing of Hypothesis Enzyme may get affected by various factors.
Such system allows re-absorption of water and critical ions from the excreted urine. Urea is reabsorbed in the inner medullary collecting ducts of the nephrons. Urea is measured as Urea Nitrogen (Urea N) and constitutes 45% of the total non-protein nitrogen. Urea N in blood or urine is an important indicator of liver and kidney function. The concentration of urea N is determined by the use of Urease.
This is what causes the buildup of amino acids (Adrian 774). In her 2010 article, “Maple Syrup Urine Disease,” in “Genetics and Inherited Conditions,” Jane Adrian explains that there are 4 genes involved. They help encode for specific proteins. The genes are BCKDHA, BCKDHB, DBT, and DLD. These proteins consist of three catalysts and two regulatory enzymes .
What is the difference between substrate level phosphorylation & oxidative phosphorylation? Substrate level phosphorylation occurs in the cytoplasm while oxidative occurs in the mitocondria. In substrate level, a phosphate group is removed directly from a subtrate and is transferred to ADP. In oxidative, electrons are transferred from NADH+ through the ETC in the mitochondria's inner membrane. 8.
What is the size difference between fat polymers and starch and protein polymers? Experiment 4: Protein Test Fill in the table below with the results from the protein and peptides test experiment.. Results Protein and Peptides Test Solution Initial Color Color with Biuret Solution egg albumin solution water What results would you expect from a biuret test of
To determine these percentages however, an indirect measurement was performed where RuBisCO is converted on a total protein basis assuming the chlorophyll: total protein mass ratio is 0.0421. Therefore, Losh et al. conducted experiments where RuBisCO was measured with Quantitative Western blots using an antibody which binds to a conserved region of the large subunit of RuBisCO. They concluded that RuBisCO represented < 6% of total protein in eight species of microalgae. Furthermore, they concluded that unlike in plants, RuBisCO does not account for a major fraction of cellular nitrogen in
In all forms of digestion (whether of proteins, carbohydrates, or fats), larger molecules are broken down into smaller molecules by a reaction with water in which a water molecule is split in two, with each part joining a different product molecule. This type of reaction is called hydrolysis. Remember that proteins are long chains of amino acids linked together by amide functional groups called peptide bonds. When protein molecules are digested, a series of hydrolysis reactions convert them into separate amino acids. RCONR2 + H2O RCO2H + HNR2 In the laboratory, the reaction above is very slow unless a strong acid catalyst is added to mixture, yet in the small intestines, where the conditions are essentially neutral rather than acidic, most of the hydrolysis of proteins takes place rather quickly.