Evolution: Bacterial Resistance Lab

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Lab 3: The Evolution of Antibiotic Resistance Abstract In this lab, the issue was on antibiotic resistance and how bacteria evolves over a short period of time to become resistant to a certain antibiotic that is administered when trying to treat an illness or infection. In order to test antibiotic resistance, three techniques were used: PCR mutagenesis to mimic the evolution in the CTX-M-2 gene by inducing mutations with in it, selecting alleles that can give resistance to an antibiotic drug, and test the resistance of the mutant alleles to other antibiotics (Barlow, 2). Observations at the end of the lab showed that there was a change in antibiotic resistance because of the induced mutations of the CTX-M-2 gene; some mutants were less resistant and some were more resistant to the antibiotics used. So, the conclusion that was derived from the final results was that mutations do not always affect how resistant a bacteria will become, but that mutations do affect how the antibiotics react to the bacteria when the two are combined. Introduction When the antibiotic penicillin was first discovered in the 1940's, it was thought to be the drug that could cure all illnesses or infectious diseases; it was able to cure pneumonia and tuberculosis. Not to long after penicillin was discovered though, it seemed to become less and less effective in treating or curing an illness. This was because the bacteria that would cause certain illnesses were rapidly developing a resistance to antibiotics that were used to fight off the bacteria. Nowadays, scientists have found ways to predict how resistant or non-resistant certain strains of bacteria will become when a certain antibiotic is tested against it. Although it seems there has been a lot of success in developing new vaccines every time a bacterial strain becomes resistant to an antibiotic, it is not an easy process. The
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