Look in the water bath on your table for a flask labeled DMA. This flask contains Davis Minimal Agar that has been autoclaved to make it sterile, and is being kept at 47 C to keep it liquefied. 3. Think about these important points in pouring a petri plate before doing it: a) You must work quickly, because once the container of minimal agar is removed from the bath, it will start to harden within 2-3 minutes. b) When pouring agar into the petri dish, pour just enough to fill the dish about half way.
· Broth culture of E. coli · Tubes of sterile nutrient broth · Inoculating loop · Bunsen burner · disinfectant Procedure 1. Disinfect the work surface by spraying it down with a disinfectant and then wipe with paper towel 2. Light Bunsen burner 3. Sterilize inoculating loop by holding it over the flame of the Bunsen burner until the tip becomes bright red. 4.
Observe after at least 5 minutes. For a closer examination, use the glass rod, popsicle stick or toothpick to hook the precipitate. * Step 6 – Pour the liquids down the sink and clean the rest of the equipment. Read more at Suite101: How to Extract DNA From Fruit and Vegetables: A Home Experiment for Viewing Real Deoxyribonkucleic Acid http://www.suite101.com/content/how-to-extract-dna-from-fruit-and-vegetables-a76806#ixzz1EkVq2LaJ Theory The mashing breaks the cells apart. The detergent breaks them open, freeing the cell contents (including the DNA).
6 grams of dried seaweed, cut into ½ inches should be places into a 150 mL beakers that will later be filled with ¾ distilled or deionized water. Then, the seaweed should be agitated with a stirring rod to remove monosodium glutamate. 2. After the water is poured off in the sink, 40 mL of distilled water should be added to the seaweed, and should be heated for 5 minutes with a Bunsen burner. 3.
* Then you will have to put the test tube in a water bath and leave it until the contents reach the same temperature as the water bath. * Then you will have to take the thermometer from the test tube and put a glass rod into it instead. * After this you will have to use a 2cm syringe to measure out 1cm of lipase to the beaker in the water for the temperature you are investigating. * Then add the lipase to the test
2. Cut the beetroot cylinders, with a knife, to exactly 3 cm. Use a ruler to measure 3 cm from the cylinder. 3. Then wash the beetroot cylinders with the use of water.
Determine what your group’s hypothesis will be and write it as an “if, then” statement on your lab report. 2. Label each petri dish with the 4 different amounts of salt using the grease pencil: (control (0%), 1%, 2% and 3%) 3. To make salt concentrations of 1%, 2% and 3%: -Place 100 mL of water in beaker -To make a concentration of 1%, add 1 gram of salt (for
Put the open end of the measuring cylinder upside down, filled with water, in the water 4. Put one end of the tube into the measuring cylinder 5. Put 20cm3 of hydrochloric acid into the boiling tube 6. Add the marble chips into the hydrochloric acid and put the other end of the tube with the rubber stopper, onto the boiling tube allowing all gas through the tube 7. Stir the solution by making a circle motion with the tube
Oil-in-Water & Water-in-Oil Emulsions Introduction In this activity we will compare water in oil emulsions and oil in water emulsions with water-soluble food coloring. Material 5 small cups Microscope slides Food coloring 5 coffee stirrers Whole milk Butter Margarine Mayonnaise Vinaigrette dressing To Do and Notice Bring butter and margarine to room temperature before the start of the activity or melt 1 tablespoon of each in the microwave for 30 seconds. Measure out approximately one tablespoon of butter, whole milk, margarine, mayonnaise, and salad dressing into 5 separate small cups. With a dropper place a few drops of food coloring onto the surface of each sample. Mix each sample with a coffee stirrer then use the stirrer to spread a thin layer of each stained sample on a microscope slide.
The watch glass was removed with the beaker tongs. Using a rubber bulb and a stirring rod to stir the solution continuously, 15.00mL of .25M BaCl2 solution was added to the solution in the beaker. The watch glass is replaced and the solution is keep hot but not boiling for 15 minutes. The precipitate was allowed to settle. When the liquid above the precipitate was clear, the solution was tested for completeness of precipitation when a few drops of BaCl2 solution were added from a pipette.